Overexpression of an Arabidopsis cDNA encoding a sterol-C241-methyltransferase in tobacco modifies the ratio of 24-methyl cholesterol to sitosterol and is associated with growth reduction

被引:60
作者
Schaller, H [1 ]
Bouvier-Navé, P [1 ]
Benveniste, P [1 ]
机构
[1] Inst Bot, Inst Biol Mol Plantes, Dept Enzymol Cellulaire & Mol, F-67083 Strasbourg, France
关键词
D O I
10.1104/pp.118.2.461
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Higher plants synthesize 24-methyl sterols and 24-ethyl sterols in defined proportions. As a first step in investigating the physiological function of this balance, an Arabidopsis cDNA encoding an S-adenosyl-L-methionine 24-methylene lophenol-C24(1)-methyltransferase, the typical plant enzyme responsible for the production of 24-ethyl sterols, was expressed in tobacco (Nicotiana tabacum L.) under the control of a constitutive promoter. Transgenic plants displayed a novel 24-alkyl-Delta(5)-sterol profile: the ratio of 24-methyl cholesterol to sitosterol, which is close to 1 in the wild type, decreased dramatically to values ranging from 0.01 to 0.31. In succeeding generations of transgenic tobacco, a high S-adenosyl-L-methionine 24-methylene lophenol-C24(1)-methyltransferase enzyme activity and, consequently, a low ratio of 24-methyl cholesterol to sitosterol, was associated with reduced growth compared with the wild type. However, this new morphological phenotype appeared only below the threshold ratio of 24-methyl cholesterol to sitosterol of approximately 0.1. Because the size of cells was unchanged in small, transgenic plants, we hypothesize that a radical decrease of 24-methyl cholesterol and/or a concomitant increase of sitosterol would be responsible for a change in cell division through as-yet unknown mechanisms.
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页码:461 / 469
页数:9
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