Multifunctional Mesoporous Nanoellipsoids for Biological Bimodal Imaging and Magnetically Targeted Delivery of Anticancer Drugs

被引:236
作者
Chen, Yu [1 ]
Chen, Hangrong [1 ]
Zhang, Shengjian [2 ]
Chen, Feng [1 ]
Zhang, Lingxia [1 ]
Zhang, Jiamin [3 ]
Zhu, Min [1 ]
Wu, Huixia [1 ]
Guo, Limin [1 ]
Feng, Jingwei [1 ]
Shi, Jianlin [1 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China
[2] Fudan Univ, Dept Radiol, Shanghai Canc Hosp, Shanghai 200032, Peoples R China
[3] Shanghai Inst Blood Transfus, Shanghai Red Cross Blood Ctr, Shanghai 200051, Peoples R China
基金
中国博士后科学基金;
关键词
SILICA NANOPARTICLES; CANCER-CELLS; INORGANIC NANOPARTICLES; POLYMERIC MICELLES; PASSIVE DIFFUSION; SHELL STRUCTURE; HOLLOW SPHERES; DOXORUBICIN; LUMINESCENT; DNA;
D O I
10.1002/adfm.201001495
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A general polyelectrolyte-mediated self-assembly technique is adopted to prepare multifunctional mesoporous nanostructures as an effective biological bimodal imaging probe and magnetically targeted anticancer drug (doxorubicin) delivery systems (DDSs). A positively charged polyelectrolyte (PAH) and negatively charged fluorescent quantum dots (QDs) are successfully assembled onto the surface of ellipsoidal Fe(3)O(4)@SiO(2)@mSiO(2) composite nanostructures to combine the merits of tunable fluorescent/magnetic properties, mesoporous nanostructures for drug loading, and the uniform ellipsoidal morphology for enhanced uptake by cancer cells. The resultant nanoellipsoids are homogeneously coated with four layers of PAH/QDs, with an additional PAH layer to make the ellipsoidal surface positively charged. This acts to enhance cellular uptake, which is driven by electrostatic interactions between the positive nanoparticle surface and the negative cell surface. The high biocompatibility of the achieved multifunctional nanoellipsoids is demonstrated by a cell-cytotoxicity assay, hemolyticity against human red blood cells, and coagulation evaluation of fresh human blood plasma after exposure to the nanoparticles. Moreover, confocal microscopy and bio-TEM observations show that the cell uptake of nanocarriers is dose-dependent, and the nanoparticles accumulate mostly in the cytoplasm. The excellent capability of the nanocarriers as contrast agents for MRI is demonstrated by the relatively high r(2) value (143 mM(-1)s(-1)) and preliminary in vivo characterization. More importantly, the doxorubicin-loaded DDSs show higher cytotoxicity than the free doxorubicin drug as contributed by the intracellular release pathway of doxorubicin from the DDSs, indicating the potential application of the obtained multifunctional mesoporous nanoellipsoids as highly effective bimodal imaging probes and DDSs for cancer diagnosis and chemotherapy, simultaneously.
引用
收藏
页码:270 / 278
页数:9
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