Metabolic characterization of Brassica rapa leaves by NMR spectroscopy

被引：83

作者：

Abdel-Farid, Brahim Bayoumi ^{[1
]}

Kim, Hye Kyong ^{[1
]}

Choi, Young Hae ^{[1
]}

Verpoorte, Robert ^{[1
]}

机构：

[1] Leiden Univ, Inst Biol, Div Pharmacognosy, Metab Sect, NL-2300 RA Leiden, Netherlands

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2007年 / 55卷 / 19期

关键词：

metabolomic analysis; NMR; principal component analysis; Brassica rapa; cultivars; biological variation; developmental stages;

D O I：

10.1021/jf071294b

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The Brassica has been intensively studied due to the nutritional and beneficial effects. However, many species, varieties, and cultivars of this genus and the resulting large metabolic variation have been obstacles for systematic research of the plant. In order to overcome the problems posed by the biological variation, the metabolomic analysis of various cultivars of Brassica rapa was performed by NMR spectroscopy combined with multivariate data analysis. Discriminating metabolites in different cultivars and development stages were elucidated by diverse 2D-NMR techniques after sorting out different significant signals using (1)H NMR measurements and principal component analysis. Among the elucidated metabolites, several organic and amino acids, carbohydrates, adenine, indole acetic acid (IAA), phenylpropanoids, flavonoids, and glucosinolates were found to be the metabolites contributing to the differentiation between cultivars and age of Brassica rapa. On the basis of these results, the distribution of plant metabolites among different cultivars and development stages of B. rapa is discussed.

引用

页码：7936 / 7943

页数：8

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