Ribozyme catalysis: not different, just worse

被引:114
作者
Doudna, JA [1 ]
Lorsch, JR
机构
[1] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Chem, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] Johns Hopkins Univ, Sch Med, Dept Biophys & Biophys Chem, Baltimore, MD 21205 USA
关键词
D O I
10.1038/nsmb932
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Evolution has resoundingly favored protein enzymes over RNA-based catalysts, yet ribozymes occupy important niches in modern cell biology that include the starring role in catalysis of protein synthesis on the ribosome. Recent results from structural and biochemical studies show that natural ribozymes use an impressive range of catalytic mechanisms, beyond metalloenzyme chemistry and analogous to more chemically diverse protein enzymes. These findings make it increasingly possible to compare details of RNA- and protein-based catalysis.
引用
收藏
页码:395 / 402
页数:8
相关论文
共 106 条
[1]   Crystal structure of a self-splicing group I intron with both exons [J].
Adams, PL ;
Stahley, MR ;
Kosek, AB ;
Wang, JM ;
Strobel, SA .
NATURE, 2004, 430 (6995) :45-50
[2]   Role of entropy in increased rates of intramolecular reactions [J].
Armstrong, AA ;
Amzel, LM .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (47) :14596-14602
[3]   Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons [J].
Asai, T ;
Condon, C ;
Voulgaris, J ;
Zaporojets, D ;
Shen, BH ;
Al-Omar, M ;
Squires, C ;
Squires, CL .
JOURNAL OF BACTERIOLOGY, 1999, 181 (12) :3803-3809
[4]   The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution [J].
Ban, N ;
Nissen, P ;
Hansen, J ;
Moore, PB ;
Steitz, TA .
SCIENCE, 2000, 289 (5481) :905-920
[5]   Self-cleaving ribozymes of hepatitis delta virus RNA [J].
Been, MD ;
Wickham, GS .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 247 (03) :741-753
[6]   Mechanistic considerations for general acid-base catalysis by RNA: Revisiting the mechanism of the hairpin ribozyme [J].
Bevilacqua, PC .
BIOCHEMISTRY, 2003, 42 (08) :2259-2265
[7]   Steric interference modification of the hammerhead ribozyme [J].
Blount, KF ;
Grover, NL ;
Mokler, V ;
Beigelman, L ;
Uhlenbeck, OC .
CHEMISTRY & BIOLOGY, 2002, 9 (09) :1009-1016
[8]   Fast cleavage kinetics of a natural hammerhead ribozyme [J].
Canny, MD ;
Jucker, FM ;
Kellogg, E ;
Khvorova, A ;
Jayasena, SD ;
Pardi, A .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (35) :10848-10849
[9]   Crystal structure of a group I ribozyme domain: Principles of RNA packing [J].
Cate, JH ;
Gooding, AR ;
Podell, E ;
Zhou, KH ;
Golden, BL ;
Kundrot, CE ;
Cech, TR ;
Doudna, JA .
SCIENCE, 1996, 273 (5282) :1678-1685
[10]   RNAS WITH DUAL-SPECIFICITY AND DUAL RNAS WITH SIMILAR SPECIFICITY [J].
CONNELL, GJ ;
YARUS, M .
SCIENCE, 1994, 264 (5162) :1137-1141