Ribozyme catalysis: not different, just worse

被引:114
作者
Doudna, JA [1 ]
Lorsch, JR
机构
[1] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Chem, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] Johns Hopkins Univ, Sch Med, Dept Biophys & Biophys Chem, Baltimore, MD 21205 USA
关键词
D O I
10.1038/nsmb932
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Evolution has resoundingly favored protein enzymes over RNA-based catalysts, yet ribozymes occupy important niches in modern cell biology that include the starring role in catalysis of protein synthesis on the ribosome. Recent results from structural and biochemical studies show that natural ribozymes use an impressive range of catalytic mechanisms, beyond metalloenzyme chemistry and analogous to more chemically diverse protein enzymes. These findings make it increasingly possible to compare details of RNA- and protein-based catalysis.
引用
收藏
页码:395 / 402
页数:8
相关论文
共 106 条
[41]   Direct pKa measurement of the active-site cytosine in a genomic hepatitis delta virus ribozyme [J].
Lupták, A ;
Ferré-D'Amaré, AR ;
Zhou, KH ;
Zilm, KW ;
Doudna, JA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (35) :8447-8452
[42]   STEREOCHEMISTRY OF RNA CLEAVAGE BY THE TETRAHYMENA RIBOZYME AND EVIDENCE THAT THE CHEMICAL STEP IS NOT RATE-LIMITING [J].
MCSWIGGEN, JA ;
CECH, TR .
SCIENCE, 1989, 244 (4905) :679-683
[43]   Structures and mechanisms of Nudix hydrolases [J].
Mildvan, AS ;
Xia, Z ;
Azurmendi, HF ;
Saraswat, V ;
Legler, PM ;
Massiah, MA ;
Gabelli, SB ;
Bianchet, MA ;
Kang, LW ;
Amzel, LM .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2005, 433 (01) :129-143
[44]   INTERACTION OF TRANSFER-RNA WITH 23S RIBOSOMAL-RNA IN THE RIBOSOMAL A-SITES, P-SITES, AND E-SITES [J].
MOAZED, D ;
NOLLER, HF .
CELL, 1989, 57 (04) :585-597
[45]   The hammerhead, hairpin and VS ribozymes are catalytically proficient in monovalent cations alone [J].
Murray, JB ;
Seyhan, AA ;
Walter, NG ;
Burke, JM ;
Scott, WG .
CHEMISTRY & BIOLOGY, 1998, 5 (10) :587-595
[46]   Does a single metal ion bridge the A-9 and scissile phosphate groups in the catalytically active hammerhead ribozyme structure? [J].
Murray, JB ;
Scott, WG .
JOURNAL OF MOLECULAR BIOLOGY, 2000, 296 (01) :33-41
[47]   A pH-dependent conformational change, rather than the chemical step, appears to be rate-limiting in the hammerhead ribozyme cleavage reaction [J].
Murray, JB ;
Dunham, CM ;
Scott, WG .
JOURNAL OF MOLECULAR BIOLOGY, 2002, 315 (02) :121-130
[48]   The structural basis of hammerhead ribozyme self-cleavage [J].
Murray, JB ;
Terwey, DP ;
Maloney, L ;
Karpeisky, A ;
Usman, N ;
Beigelman, L ;
Scott, WG .
CELL, 1998, 92 (05) :665-673
[49]   PRINCIPLES FOR THE CONSTRUCTION AND OPERATION OF A DEVICE FOR RAPIDLY FREEZING SUSPENSIONS FOR CRYOELECTRON MICROSCOPY [J].
MURRAY, JM ;
WARD, R .
JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1987, 5 (03) :279-284
[50]   A single adenosine with a neutral pKa in the ribosomal peptidyl transferase center [J].
Muth, GW ;
Ortoleva-Donnelly, L ;
Strobel, SA .
SCIENCE, 2000, 289 (5481) :947-950