Detection and characterization of phospholipase D by flow injection analysis

被引:9
作者
Becker, M
Spohn, U
UlbrichHofmann, R
机构
[1] Martin-Luther-Univ. Halle/Wittenberg, Institut für Biotechnologie, D-06120 Halle
关键词
D O I
10.1006/abio.1996.9864
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A precisely working automated system for the investigation of phospholipases D (PLDs, EC 3.1.4.4) from plant and microbial sources with how injection analysis (FIA) has been developed. The two versions of the FIA setup described are based on the oxidation of choline liberated from phosphatidylcholine by PLD action and catalyzed by choline oxidase and the chemiluminescence detection of hydrogen peroxide produced by this reaction. The correlation between this chemiluminescence signal and the PLD activity was linear in the range between 1 and 100 mU/ml PLD. The sampling frequency was 12 samples per hour. This method was used to compare three different PLDs from cabbage and microbial sources with respect to their pH optima, temperature stability, effecters, and v/[S] characteristics. (C) 1997 Academic Press, Inc.
引用
收藏
页码:55 / 61
页数:7
相关论文
共 56 条
[1]   Selective determination of gas dialysable components in complex sample solutions using triangle programmed coulometric titration in continuous flow systems. [J].
Becker, M ;
Fuhrmann, B ;
Spohn, U .
ANALYTICA CHIMICA ACTA, 1996, 324 (2-3) :115-123
[2]   INVOLVEMENT OF A PHOSPHOLIPASE-D IN THE MECHANISM OF ACTION OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR (GM-CSF) - PRIMING OF HUMAN NEUTROPHILS INVITRO WITH GM-CSF IS ASSOCIATED WITH ACCUMULATION OF PHOSPHATIDIC-ACID AND DIRADYLGLYCEROL [J].
BOURGOIN, S ;
PLANTE, E ;
GAUDRY, M ;
NACCACHE, PH ;
BORGEAT, P ;
POUBELLE, PE .
JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (03) :767-777
[3]   ENZYME SENSOR FOR THE DETERMINATION OF CHOLINE-CONTAINING PHOSPHOLIPIDS IN SOME BIOLOGICAL-FLUIDS [J].
CAMPANELLA, L ;
TOMASSETTI, M ;
SAMMARTINO, MP .
ANALYST, 1988, 113 (01) :77-80
[4]   A SIMPLE ASSAY FOR THE QUANTITATIVE-EVALUATION OF PHOSPHOLIPASE-D ACTIVITY [J].
CARDILLO, R ;
DARRIGO, P ;
DEFERRA, L ;
PIERGIANNI, V ;
SCARCELLI, D ;
SERVI, S .
BIOTECHNOLOGY TECHNIQUES, 1993, 7 (11) :795-798
[5]   AN ELECTROCHEMICAL IMMUNOASSAY FOR CLOSTRIDIUM-PERFRINGENS PHOSPHOLIPASE-C [J].
CARDOSI, M ;
BIRCH, S ;
TALBOT, J ;
PHILLIPS, A .
ELECTROANALYSIS, 1991, 3 (03) :169-176
[6]  
CI YX, 1990, ANAL CHIM ACTA, V237, P497
[7]   CARDIOLIPIN SPECIFIC PHOSPHOLIPASE D ACTIVITY IN ESCHERICHIA-COLI EXTRACTS [J].
COLE, R ;
BENNS, G ;
PROULX, P .
BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 337 (03) :325-332
[8]  
DAHL JL, 1974, ANNU REV BIOCHEM, V43, P234
[9]   DETERMINATION OF FREE CHOLINE IN PLASMA AND ERYTHROCYTE SAMPLES AND CHOLINE DERIVED FROM MEMBRANE PHOSPHATIDYLCHOLINE BY A CHEMILUMINESCENCE METHOD [J].
DAS, I ;
DEBELLEROCHE, J ;
MOORE, CJ ;
ROSE, FC .
ANALYTICAL BIOCHEMISTRY, 1986, 152 (01) :178-182
[10]  
DAWSON R M C, 1966, Colloquium der Gesellschaft fuer Physiologische Chemie, V16, P29