Structure characterization of polysaccharide isolated from the fruiting bodies of Tricholoma matsutake

被引：85

作者：

Ding, Xiang ^{[1
]}

Feng, Su ^{[1
]}

Cao, Mei ^{[2
]}

Li, Mao-tao ^{[1
]}

Tang, Jie ^{[1
]}

Guo, Chun-xiao ^{[1
]}

Zhang, Jie ^{[1
]}

Sun, Qun ^{[1
]}

Yang, Zhi-rong ^{[1
]}

Zhao, Jian ^{[1
,2
]}

机构：

[1] Sichuan Univ, Coll Life Sci, Minist Educ, Key Lab Biol Resource & Ecol Environm, Chengdu 610064, Peoples R China

[2] Sichuan Prov Peoples Hosp, Key Lab Sichuan Acad Med Sci, Chengdu 610072, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 81卷 / 04期

关键词：

Monosaccharide component; Polysaccharide structure; GC MS; C-13 and H-1 NMR; Tricholoma matsutake; ANTIOXIDANT ACTIVITIES; EXTRACTS; ALKALINE; GLUCANS; CELL; ROOT;

D O I：

10.1016/j.carbpol.2010.04.010

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A novel heteropolysaccharide was isolated from the fruiting bodies of Tricholoma matsutake through DEAF-cellulose column and Sephadex G-100 column. The T. matsutake polysaccharide (TMP-A) had a molecular weight of 8.89 x 10(4) Da and was mainly composed of 4-D-Glc:4,6-D-Glc:3-D-Gal:T-D-Xyl and with the ratio of 7:1:1:1. The structural features of TMP-A were investigated by a combination of total hydrolysis, methylation analysis, gas chromatography-mass spectrometry (GC-MS), scanning electron microscope (SEM), infrared (IR) spectra, nuclear magnetic resonance (NMR) spectroscopy and dynamical analysis of the atomic force microscope (AFM) studies. The results indicated that TMP-A had a backbone of 1,4-beta-glucopyranos which branches at 0-6 composed of an (1 -> 3)-alpha-galactopyranose residue and terminated with alpha-xylopyranose residue. Chain conformation study showed that the polysaccharide took random coil compact conformation. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：942 / 947

页数：6

共 44 条

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