Coherent scattering in multi-harmonic light microscopy

被引：211

作者：

Moreaux, L

Sandre, O

Charpak, S

Blanchard-Desce, M

Mertz, J

机构：

[1] ESPCI, INSERM EPI 00 02, Neurophysiol Lab, F-75005 Paris, France

[2] Inst Curie, Lab Physicochim, CNRS, UMR 168, Paris, France

[3] Univ Rennes, CNRS UMR 6510, Lab Synthese & Electrosynthese Organ, Rennes, France

来源：

BIOPHYSICAL JOURNAL | 2001年 / 80卷 / 03期

关键词：

D O I：

10.1016/S0006-3495(01)76129-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

By focusing a pulsed laser beam into a sample, harmonic up-conversion can be generated as well as multi-photon excited fluorescence. Whereas multi-photon excited fluorescence microscopy is well established, the use of multi-harmonic generation for three-dimensional image contrast is very recent. Both techniques can provide similar resolution and, for adequate radiating source density, comparable signal levels, allowing them to be combined in a single versatile instrument.; However, harmonic generation differs fundamentally from fluorescence generation in that it is coherent and produces radiation patterns that are highly sensitive to phase. As such, multi-harmonic generation microscopy provides a unique window into molecular spatial organization that is inaccessible to fluorescence.

引用

页码：1568 / 1574

页数：7

共 39 条

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