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Evaluation of seaFAST, a rapid fluorescent in situ hybridization test, for detection of Helicobacter pylori and resistance to clarithromycin in paraffin-embedded biopsy sections

被引：30
作者：
Morris, JM
Reasonover, AL
Bruce, MG
Bruden, DL
McMahon, BJ
Sacco, FD
Berg, DE
Parkinson, AJ
机构：
[1] CDC, Arctic Invest Program, Anchorage, AK 99508 USA
[2] Alaska Native Tribal Hlth Consortium, Anchorage, AK USA
[3] Washington Univ, Sch Med, St Louis, MO 63130 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 07期
关键词：
D O I：
10.1128/JCM.43.7.3494-3496.2005
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A commercially available rapid fluorescent in situ hybridization (FISH) test, (seaFAST H. pylori Combi-Kit; SeaPro Theranostics International, Lelystad, The Netherlands) was used to simultaneously detect the presence of Helicobacter pylori and determine clarithromycin susceptibility in paraffin-embedded biopsy sections. The FISH method was found to be 97% sensitive, 94% specific for the detection of H. pylori and comparable to agar dilution for the detection of resistance to clarithromycin.
引用
收藏
页码：3494 / 3496
页数：3
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