Mining human antibody repertoires

被引：37

作者：

Beerli, Roger R. ^{[1
]}

Rader, Christoph ^{[2
]}

机构：

[1] Cytos Biotechnol AG, Schlieren, Switzerland

[2] NCI, Expt Transplantat & Immunol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

来源：

MABS | 2010年 / 2卷 / 04期

基金：

美国国家卫生研究院;

关键词：

human monoclonal antibodies; B cells; hybridoma technology; display technologies; antibody libraries; antibody engineering; HUMAN MONOCLONAL-ANTIBODIES; HIGH-AFFINITY ANTIBODIES; SINGLE-CHAIN FV; MEMORY B-CELLS; COMPLEMENTARITY-DETERMINING REGIONS; PHAGE DISPLAY LIBRARIES; FULLY HUMAN-ANTIBODIES; HUMAN PERIPHERAL-BLOOD; YEAST SURFACE DISPLAY; IN-VITRO SELECTION;

D O I：

10.4161/mabs.12187

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Human monoclonal antibodies (mAbs) have become drugs of choice for the management of an increasing number of human diseases. Human antibody repertoires provide a rich source for human mAbs. Here we review the characteristics of natural and non-natural human antibody repertoires and their mining with non-combinatorial and combinatorial strategies. In particular, we discuss the selection of human mAbs from naive, immune, transgenic and synthetic human antibody repertoires using methods based on hybridoma technology, clonal expansion of peripheral B cells, single-cell PCR, phage display, yeast display and mammalian cell display. Our reliance on different strategies is shifting as we gain experience and refine methods to the efficient generation of human mAbs with superior pharmacokinetic and pharmacodynamic properties.

引用

收藏

页码：365 / 378

页数：14

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