Fusobacterium nucleatum apoptosis-inducing outer membrane protein

被引:37
作者
Kaplan, CW
Lux, R
Huynh, T
Jewett, A
Shi, W
Haake, SK
机构
[1] Univ Calif Los Angeles, Sch Dent, Dept Oral Biol & Oral Med, Dent Res Inst,Sect Periodont, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Sch Dent, Inst Mol Biol, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Sch Dent, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA
关键词
Fusobacterium nucleatum; apoptosis; autotransporter; aim1; mutant;
D O I
10.1177/154405910508400803
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The periodontal pathogen Fusobacterium nucleatum induces apoptosis in lymphocytes. We previously identified the autotransporter protein Fap2 in F. nucleatum strain PK1594 that induced apoptosis in lymphocytes when expressed in Escherichia coli. In this study, we identified protein homologs of Fap2 in the transformable F. nucleatum strain ATCC 23726, to determine their role in the induction of apoptosis in lymphocytes. We used a new gene-inactivation vector conferring thiamphenicol resistance (pHS31) to construct a mutant deficient in one of the homologs, aim1. Transcriptional analyses demonstrated disruption of aim1 expression, and phenotypic analyses revealed a 41% decrease in the ability of the mutant to induce apoptosis in Jurkat cells, as compared with the parental strain. These studies demonstrate, in the native host cell background, the contribution of aim1 to F. nucleatum induction of apoptosis and, to the best of our knowledge, represent the first report of a genetically defined and phenotypically characterized mutation in F. nucleatum.
引用
收藏
页码:700 / 704
页数:5
相关论文
共 23 条
[11]   Genome analysis of F-nucleatum sub spp vincentii and its comparison with the genome of F-nucleatum ATCC 25586 [J].
Kapatral, V ;
Ivanova, N ;
Anderson, I ;
Reznik, G ;
Bhattacharyya, A ;
Gardner, WL ;
Mikhailova, N ;
Lapidus, A ;
Larsen, N ;
D'Souza, M ;
Walunas, T ;
Haselkorn, R ;
Overbeek, R ;
Kyrpides, N .
GENOME RESEARCH, 2003, 13 (06) :1180-1189
[12]   Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586 [J].
Kapatral, V ;
Anderson, I ;
Ivanova, N ;
Reznik, G ;
Los, T ;
Lykidis, A ;
Bhattacharyya, A ;
Bartman, A ;
Gardner, W ;
Grechkin, G ;
Zhu, LH ;
Vasieva, O ;
Chu, L ;
Kogan, Y ;
Chaga, O ;
Goltsman, E ;
Bernal, A ;
Larsen, N ;
D'Souza, M ;
Walunas, T ;
Pusch, G ;
Haselkorn, R ;
Fonstein, M ;
Kyrpides, N ;
Overbeek, R .
JOURNAL OF BACTERIOLOGY, 2002, 184 (07) :2005-2018
[13]   A unique nine-gene comY operon in Streptococcus mutans [J].
Merritt, J ;
Qi, FX ;
Shi, WY .
MICROBIOLOGY-SGM, 2005, 151 :157-166
[14]   BACTERIOLOGY OF SEVERE PERIODONTITIS IN YOUNG-ADULT HUMANS [J].
MOORE, WEC ;
HOLDEMAN, LV ;
SMIBERT, RM ;
HASH, DE ;
BURMEISTER, JA ;
RANNEY, RR .
INFECTION AND IMMUNITY, 1982, 38 (03) :1137-1148
[15]   COMPARATIVE BACTERIOLOGY OF JUVENILE PERIODONTITIS [J].
MOORE, WEC ;
HOLDEMAN, LV ;
CATO, EP ;
SMIBERT, RM ;
BURMEISTER, JA ;
PALCANIS, KG ;
RANNEY, RR .
INFECTION AND IMMUNITY, 1985, 48 (02) :507-519
[16]   BACTERIOLOGY OF EXPERIMENTAL GINGIVITIS IN YOUNG-ADULT HUMANS [J].
MOORE, WEC ;
HOLDEMAN, LV ;
SMIBERT, RM ;
GOOD, IJ ;
BURMEISTER, JA ;
PALCANIS, KG ;
RANNEY, RR .
INFECTION AND IMMUNITY, 1982, 38 (02) :651-667
[17]  
MOORE WEC, 1984, INFECT IMMUN, V46, P1
[18]   THE ROLE OF ANAEROBIC-BACTERIA IN THE PATHOGENESIS OF URINARY-TRACT INFECTIONS [J].
RIBOT, S ;
GAL, K ;
GOLDBLAT, MV ;
ESLAMI, HH .
JOURNAL OF UROLOGY, 1981, 126 (06) :852-853
[19]   ANAEROBIC BACTERIA IN ORAL INFECTIONS [J].
SABISTON, CB ;
GOLD, WA .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 1974, 38 (02) :187-192
[20]   CAPACITY OF ANAEROBIC-BACTERIA FROM NECROTIC DENTAL PULPS TO INDUCE PURULENT INFECTIONS [J].
SUNDQVIST, GK ;
ECKERBOM, MI ;
LARSSON, AP ;
SJOGREN, UT .
INFECTION AND IMMUNITY, 1979, 25 (02) :685-693