Determination of free proline and monosaccharides in wine samples by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD)

被引:34
作者
Cataldi, TRI [1 ]
Nardiello, D [1 ]
机构
[1] Univ Basilicata, Dipartimento Chim, I-85100 Potenza, Italy
关键词
wine; must; grapes; sugars; proline; chromatography; amperometric detection;
D O I
10.1021/jf034069c
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
A sensitive and selective analytical method for the simultaneous separation and quantitative determination of proline and free monosaccharides in wine samples by high-performance anion-exchange chromatography coupled with pulsed amperometric detection is described. Under optimized experimental conditions, a complete separation was obtained in less than 30 min, using an isocratic elution with 10 mM NaOH and 1 mM Ba(OAc)(2). No postcolumn addition of strong bases to the eluent for enhancing detection sensitivity was needed. Upon 25-fold sample dilution and purification to avoid interference of tannins, pigments, and phenolic compounds, the fingerprinting of common monosaccharides (i.e., arabinose, glucose, fructose, galactose, and xylose) and proline in wines, musts, and vinegars can be easily accomplished. The method allows high recovery and satisfies the necessary requirements for accuracy, repeatability, and sensitivity. Values obtained for proline content ranged from 470 to 1190 mg/L in "Aglianico" red wines (mean value, 870 +/- 192 mg/L, n = 21) and from 168 to 286 mg/L in white wines (mean value, 208 +/- 32 mg/L, n = 11). Lower levels were found in musts of red and white grapes, 550 and 87 mg/L, respectively. The lowest content of proline, ca. 10 mg/L, was found both in white and red vinegars.
引用
收藏
页码:3737 / 3742
页数:6
相关论文
共 40 条
[1]   PULSED ELECTROCHEMICAL DETECTION OF PENICILLINS USING 3-STEP AND 4-STEP WAVE-FORMS [J].
ALTUNATA, S ;
EARLEY, RL ;
MOSSMAN, DM ;
WELCH, LE .
TALANTA, 1995, 42 (01) :17-25
[2]   Application of quality control methods for assessing wine authenticity: Use of multivariate analysis (chemometrics) [J].
Arvanitoyannis, IS ;
Katsota, MN ;
Psarra, EP ;
Soufleros, EH ;
Kallithraka, S .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 1999, 10 (10) :321-336
[3]   HPLC analysis of carbohydrates in wines and instant coffees using anion exchange chromatography coupled to pulsed amperometric detection [J].
Bernal, JL ;
DelNozal, MJ ;
Toribio, L ;
DelAlamo, M .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (02) :507-511
[4]  
Calabrese M, 1999, J SCI FOOD AGR, V79, P1357, DOI 10.1002/(SICI)1097-0010(199908)79:11<1357::AID-JSFA371>3.0.CO
[5]  
2-3
[6]   Isocratic separations of closely-related mono- and disaccharides by high-performance anion-exchange chromatography with pulsed amperometric detection using dilute alkaline spiked with barium acetate [J].
Cataldi, TRI ;
Campa, C ;
Angelotti, M ;
Bufo, SA .
JOURNAL OF CHROMATOGRAPHY A, 1999, 855 (02) :539-550
[7]   Role of barium ions in the anion-exchange chromatographic separation of carbohydrates with pulsed amperometric detection [J].
Cataldi, TRI ;
Campa, C ;
Margiotta, G ;
Bufo, SA .
ANALYTICAL CHEMISTRY, 1998, 70 (18) :3940-3945
[8]   Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection: The potential is still growing [J].
Cataldi, TRI ;
Campa, C ;
De Benedetto, GE .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 2000, 368 (08) :739-758
[9]   An integrated amperometry waveform for the direct, sensitive detection of amino acids and amino sugars following anion-exchange chromatography [J].
Clarke, AP ;
Jandik, P ;
Rocklin, RD ;
Liu, Y ;
Avdalovic, N .
ANALYTICAL CHEMISTRY, 1999, 71 (14) :2774-2781
[10]   SYNTHESIS OF A FLUORESCENT DERIVATIZING REAGENT, 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE, AND ITS APPLICATION FOR THE ANALYSIS OF HYDROLYSATE AMINO-ACIDS VIA HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
COHEN, SA ;
MICHAUD, DP .
ANALYTICAL BIOCHEMISTRY, 1993, 211 (02) :279-287