Evaluation of Legionella V-TesT for the detection of Legionella pneumophila antigen in urine samples

被引：8

作者：

Bruin, J. P. ^{[1
]}

Peeters, M. F. ^{[2
]}

IJzerman, E. P. F. ^{[1
]}

Diederen, B. M. W. ^{[1
]}

机构：

[1] Reg Publ Hlth Lab, Haarlem, Netherlands

[2] St Elizabeth Hosp, Lab Med Microbiol & Immunol, Tilburg, Netherlands

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2010年 / 29卷 / 07期

关键词：

LEGIONNAIRES-DISEASE; SEROGROUP-1; ANTIGEN; INFECTION; PNEUMONIA; DIAGNOSIS;

D O I：

10.1007/s10096-010-0932-0

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We evaluated a new immunochromatographic assay (Legionella V-TesT, Coris BioConcept, Gembloux, Belgium) for its ability to detect Legionella pneumophila serogroup 1 antigen in urine. Test devices were read at various time points to determine the optimum incubation time regarding performance. The results were compared with those obtained with the BinaxNOW urinary antigen test. The sensitivity and specificity were 82.2% and 98.6%, respectively, for the Legionella V-TesT and 83.9% and 100%, respectively, for the BinaxNOW urinary antigen test after 15 min of incubation. When tests were examined after 60 min, the sensitivity for both tests increased to 91.5%.

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收藏

页码：899 / 900

页数：2

相关论文

共 10 条

[1]

Breiman R F, 1998, Semin Respir Infect, V13, P84

[2] Diagnosis of Legionella infection in Legionnaires' disease [J].

Den Boer, JW ;

Yzerman, EPF .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2004, 23 (12) :871-878

[3] Legionella spp. and Legionnaires' disease [J].

Diederen, B. M. W. .

JOURNAL OF INFECTION, 2008, 56 (01) :1-12

[4] Evaluation of the SAS Legionella Test, a new immunochromatographic assay for the detection of Legionella pneumophila serogroup 1 antigen in urine [J].

Diederen, B. M. W. ;

Peeters, M. F. .

CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (01) :86-88

[5] Evaluation of two new immunochromatographic assays (Rapid U Legionella antigen test and SD Bioline Legionella antigen test) for detection of Legionella pneumophila serogroup 1 antigen in urine [J].

Diederen, Bram M. W. ;

Peeters, Marcel F. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (08) :2991-2993

[6] Legionella and Legionnaires' disease:: 25 years of investigation [J].

Fields, BS ;

Benson, RF ;

Besser, RE .

CLINICAL MICROBIOLOGY REVIEWS, 2002, 15 (03) :506-+

[7] Comparison of diagnostic sensitivities of three assays (Bartels enzyme immunoassay [EIA], Biotest EIA, and Binax NOW immunochromatographic test) for detection of Legionella pneumophila serogroup 1 antigen in urine [J].

Guerrero, C ;

Toldos, CM ;

Yagüe, G ;

Ramírez, C ;

Rodríguez, T ;

Segovia, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (01) :467-468

[8] Delay in appropriate therapy of Legionella pneumonia associated with increased mortality [J].

Heath, CH ;

Grove, DI ;

Looke, DFM .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1996, 15 (04) :286-290

[9] Diagnosis of Legionella infection [J].

Murdoch, DR .

CLINICAL INFECTIOUS DISEASES, 2003, 36 (01) :64-69

[10] Value of intensive diagnostic microbiological investigation in low- and high-risk patients with community-acquired pneumonia [J].

van der Eerden, MM ;

Vlaspolder, F ;

de Graaff, CS ;

Groot, T ;

Jansen, HM ;

Boersma, WG .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2005, 24 (04) :241-249