Chemical synthesis of the mouse mitochondrial genome

被引：250

作者：

Gibson, Daniel G. ^{[1
]}

Smith, Hamilton O. ^{[2
]}

Hutchison, Clyde A., III ^{[2
]}

Venter, J. Craig ^{[1
,2
]}

Merryman, Chuck ^{[1
]}

机构：

[1] J Craig Venter Inst, Rockville, MD USA

[2] J Craig Venter Inst, San Diego, CA USA

来源：

NATURE METHODS | 2010年 / 7卷 / 11期

关键词：

GENE SYNTHESIS; DNA; AUTOMATION;

D O I：

10.1038/nmeth.1515

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

引用

页码：901 / U905

页数：5

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