Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

被引：1590

作者：

Gibson, Daniel G. ^{[1
]}

Glass, John I. ^{[1
]}

Lartigue, Carole ^{[1
]}

Noskov, Vladimir N. ^{[1
]}

Chuang, Ray-Yuan ^{[1
]}

Algire, Mikkel A. ^{[1
]}

Benders, Gwynedd A. ^{[2
]}

Montague, Michael G. ^{[1
]}

Ma, Li ^{[1
]}

Moodie, Monzia M. ^{[1
]}

Merryman, Chuck ^{[1
]}

Vashee, Sanjay ^{[1
]}

Krishnakumar, Radha ^{[1
]}

Assad-Garcia, Nacyra ^{[1
]}

Andrews-Pfannkoch, Cynthia ^{[1
]}

Denisova, Evgeniya A. ^{[1
]}

Young, Lei ^{[1
]}

Qi, Zhi-Qing ^{[1
]}

Segall-Shapiro, Thomas H. ^{[1
]}

Calvey, Christopher H. ^{[1
]}

Parmar, Prashanth P. ^{[1
]}

Hutchison, Clyde A., III ^{[2
]}

Smith, Hamilton O. ^{[2
]}

Venter, J. Craig ^{[1
,2
]}

机构：

[1] J Craig Venter Inst, Rockville, MD 20850 USA

[2] J Craig Venter Inst, San Diego, CA 92121 USA

来源：

SCIENCE | 2010年 / 329卷 / 5987期

关键词：

MYCOPLASMA-GENITALIUM GENOME; MINIMAL GENOME; DNA FRAGMENTS; YEAST; CLONING; GENES;

D O I：

10.1126/science.1190719

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.

引用

页码：52 / 56

页数：5

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