A role for pectin de-methylesterification in a developmentally regulated growth acceleration in dark-grown Arabidopsis hypocotyls

被引：141

作者：

Pelletier, Sandra ^{[1
,2
]}

Van Orden, Juergen ^{[3
]}

Wolf, Sebastian ^{[1
]}

Vissenberg, Kris ^{[3
]}

Delacourt, Julien ^{[1
]}

Ndong, Yves Assoumou ^{[4
]}

Pelloux, Jerome ^{[4
]}

Bischoff, Volker ^{[1
]}

Urbain, Aurelie ^{[1
]}

Mouille, Gregory ^{[1
]}

Lemonnier, Gaetan ^{[2
]}

Renou, Jean-Pierre ^{[2
]}

Hoefte, Herman ^{[1
]}

机构：

[1] INRA Ctr Versailles Grignon, INRA AgroParisTech UMR1318, Inst Jean Pierre Bourgin, F-78026 Versailles, France

[2] CNRS, INRA, Unite Mixte Rech Genom Vegetale, F-91057 Evry, France

[3] Univ Antwerp, Dept Biol, BE-2020 Antwerp, Belgium

[4] Univ Picardie Jules Verne, Unite Rech BioPI EA3900, Fac Sci, F-80039 Amiens, France

来源：

NEW PHYTOLOGIST | 2010年 / 188卷 / 03期

关键词：

Arabidopsis thaliana; cell elongation; cell wall; cellulose; Fourier transform infrared (FT-IR) microspectroscopy; hypocotyl; pectin de-methylesterification; transcriptome; CELL ELONGATION; MICROARRAY DATA; SEQUENCE TAGS; FT-IR; METHYLESTERASE; RESISTANCE; PROTEIN; ROOTS; AUXIN; ESTERIFICATION;

D O I：

10.1111/j.1469-8137.2010.03409.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

P>We focused on a developmentally regulated growth acceleration in the dark-grown Arabidopsis hypocotyl to study the role of changes in cell wall metabolism in the control of cell elongation. To this end, precise transcriptome analysis on dissected dark-grown hypocotyls, Fourier transform infrared (FT-IR) microspectroscopy and kinematic analysis were used. Using a cellulose synthesis inhibitor, we showed that the growth acceleration marks a developmental transition during which growth becomes uncoupled from cellulose synthesis. We next investigated the cellular changes that take place during this transition. FT-IR microspectroscopy revealed significant changes in cell wall composition during, but not after, the growth acceleration. Transcriptome analysis suggested a role for cell wall remodeling, in particular pectin modification, in this growth acceleration. This was confirmed by the overexpression of a pectin methylesterase inhibitor, which caused a delay in the growth acceleration. This study shows that the acceleration of cell elongation marks a developmental transition in dark-grown hypocotyl cells and supports a role for pectin de-methylesterification in the timing of this event.

引用

页码：726 / 739

页数：14

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