A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures

被引:85
作者
Duckworth, Benjamin P.
Chen, Yan
Wollack, James W.
Sham, Yuk
Mueller, Joachim D.
Taton, T. Andrew
Distefano, Mark D. [1 ]
机构
[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Phys, Minneapolis, MN 55455 USA
[3] Univ Minnesota, Minnesota Supercomp Inst, Minneapolis, MN 55455 USA
关键词
click reactions; enzymes; nanostructures; protein labeling; protein structures;
D O I
10.1002/anie.200701942
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
(Figure Presented) Protein patterning: A protein, selectively labeled with an azide tag, was reacted with alkyne-functionalized DNA. These protein-DNA conjugates were used to create a DNA-assembled protein nanostructure. This method should facilitate the preparation of a range of protein-DNA hybrid structures with useful structural and functional features. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码:8819 / 8822
页数:4
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