Proteomics of the nervous system

被引:100
作者
Husi, H [1 ]
Grant, SGN [1 ]
机构
[1] Univ Edinburgh, Dept Neurosci, Edinburgh EH9 9JZ, Midlothian, Scotland
关键词
D O I
10.1016/S0166-2236(00)01792-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The recent success of large-scale industrialized genomic sequencing opens new doors in studies of biological systems. In the current post-genomic era we must ask how to translate this DNA sequence information into an understanding of living cells, tissues and organisms. One of the major goals is to characterize protein function, biochemical pathways and networks. Achieving this aim is greatly advanced by application of new proteomic tools combined with database mining. Neuroscience in particular is poised to benefit from these approaches in light of its high complexity and cross-talk between different neurotransmitter receptors within the same synapse or across the synaptic cleft. Little is known about the global in vivo protein interactions within synapses, and the knowledge of all proteins present in such structures will help in determining sub-complexes and the modular arrangement of proteins within them. This article reviews the impact of and outlines the application of proteomic analysis in the field of neuroscience, illustrating this with the example of NMDA receptor complexes.
引用
收藏
页码:259 / 266
页数:8
相关论文
共 58 条
  • [11] Large-scale identification of secreted and membrane-associated gene products using DNA microarrays
    Diehn, M
    Eisen, MB
    Botstein, D
    Brown, PO
    [J]. NATURE GENETICS, 2000, 25 (01) : 58 - 62
  • [12] Protein function in the post-genomic era
    Eisenberg, D
    Marcotte, EM
    Xenarios, I
    Yeates, TO
    [J]. NATURE, 2000, 405 (6788) : 823 - 826
  • [13] Large-scale functional analysis using peptide or protein arrays
    Emili, AQ
    Cagney, G
    [J]. NATURE BIOTECHNOLOGY, 2000, 18 (04) : 393 - 397
  • [14] A NOVEL GENETIC SYSTEM TO DETECT PROTEIN PROTEIN INTERACTIONS
    FIELDS, S
    SONG, OK
    [J]. NATURE, 1989, 340 (6230) : 245 - 246
  • [15] Fountoulakis M, 1999, ELECTROPHORESIS, V20, P3572
  • [16] PDZ domains in synapse assembly and signalling
    Garner, CC
    Nash, J
    Huganir, RL
    [J]. TRENDS IN CELL BIOLOGY, 2000, 10 (07) : 274 - 280
  • [17] Synaptic PDZ domain-containing proteins
    Hata, Y
    Nakanishi, H
    Takai, Y
    [J]. NEUROSCIENCE RESEARCH, 1998, 32 (01) : 1 - 7
  • [18] IDENTIFYING PROTEINS FROM 2-DIMENSIONAL GELS BY MOLECULAR MASS SEARCHING OF PEPTIDE-FRAGMENTS IN PROTEIN-SEQUENCE DATABASES
    HENZEL, WJ
    BILLECI, TM
    STULTS, JT
    WONG, SC
    GRIMLEY, C
    WATANABE, C
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (11) : 5011 - 5015
  • [19] Antigen arrays in T cell immunology
    Hiemstra, HS
    Drijfhout, JW
    Roep, BO
    [J]. CURRENT OPINION IN IMMUNOLOGY, 2000, 12 (01) : 80 - 84
  • [20] Phage display of cDNA repertoires:: the pVI display system and its applications for the selection of immunogenic ligands
    Hufton, SE
    Moerkerk, PT
    Meulemans, EV
    de Bruïne, A
    Arends, JW
    Hoogenboom, HR
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1999, 231 (1-2) : 39 - 51