Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging

被引：97

作者：

Miller, Evan W. ^{[1
]}

Zeng, Li ^{[1
]}

Domaille, Dylan W. ^{[1
]}

Chang, Christopher J. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

来源：

NATURE PROTOCOLS | 2006年 / 1卷 / 02期

关键词：

D O I：

10.1038/nprot.2006.140

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Coppersensor-1 (CS1) is a small-molecule, membrane-permeable fluorescent dye for imaging labile copper pools in biological samples, including live cells. This probe, comprising a boron dipyrromethene (BODIPY) chromophore coupled to a thioether-rich receptor, has a picomolar affinity for Cu+ with high selectivity over competing cellular metal ions. CS1 fluorescence increases up to 10-fold on binding to Cu+. In this protocol we describe the synthesis of CS1 and how to use this chemical tool to investigate intracellular levels of labile copper in cultured cells. The preparation of CS1 is anticipated to take 4-5 d, and imaging assays can be performed in 1-2 d with cultured cells.

引用

页码：824 / 827

页数：4

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