Dithiobissuccinimidyl propionate self assembled monolayer based cholesterol biosensor

被引:27
作者
Arya, Sunil K.
Pandey, Pratibha
Singh, Surinder P.
Datta, Monika
Malhotra, Bansi D.
机构
[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India
[2] Univ Delhi, Dept Chem, Delhi 110007, India
关键词
D O I
10.1039/b707000d
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A dithiobissuccinimidyl propionate (DTSP) self-assembled monolayer (SAM) prepared onto a gold (Au) surface has been utilized for covalent immobilization of cholesterol oxidase (ChOx) and cholesterol esterase (ChEt). These ChOx-ChEt/DTSP/Au bio-electrodes have been characterized using electrochemical impedance and cyclic voltammetric (CV) techniques, respectively. Differential pulse voltammetry (DPV) has been used for enzymatic assay of immobilized ChOx and ChEt onto the DTSP modified gold surface as a function of cholesterol oleate concentration. The response measurement conducted on ChOx-ChEt/DTSP/Au bio-electrode reveal the value of Michaelis-Menten constant ( Km) as 0.95 mM suggesting enhanced affinity of enzymes (ChOx and ChEt). The ChOx-ChEt/DTSP/Au bio-electrodes show linearity in range of 50 to 400 mg dl(-1) of cholesterol oleate and the shelf-life of more than 50 days when stored at 4 degrees C. This biosensing electrode shows correlation coefficient of 0.9973 and standard deviation of regression as 0.859 mA.
引用
收藏
页码:1005 / 1009
页数:5
相关论文
共 36 条
  • [31] Amperometric choline biosensor fabricated through electrostatic assembly of bienzyme/polyelectrolyte hybrid layers on carbon nanotubes
    Wang, J
    Liu, GD
    Lin, YH
    [J]. ANALYST, 2006, 131 (04) : 477 - 483
  • [32] Electrochemical detection of DNA immobilized on gold colloid particles modified self-assembled monolayer electrode with silver nanoparticle label
    Wang, MJ
    Sun, CY
    Wang, LY
    Ji, XH
    Bai, YB
    Li, TJ
    Li, JH
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2003, 33 (05) : 1117 - 1125
  • [33] Stepwise surface regeneration of electrochemical immunosensors working on biocatalyzed precipitation
    Yoon, HC
    Ko, JS
    Yang, H
    Kim, YT
    [J]. ANALYST, 2002, 127 (12) : 1576 - 1579
  • [34] An impedance array biosensor for detection of multiple antibody-antigen interactions
    Yu, Xiaobo
    Lv, Renji
    Ma, Zhenqiu
    Liu, Zhihong
    Hao, Yanhong
    Li, Qingzhang
    Xu, Danke
    [J]. ANALYST, 2006, 131 (06) : 745 - 750
  • [35] Multilayer membranes for glucose biosensing via layer-by-layer assembly of multiwall carbon nanotubes and glucose oxidase
    Zhao, HT
    Ju, HX
    [J]. ANALYTICAL BIOCHEMISTRY, 2006, 350 (01) : 138 - 144
  • [36] A wireless magnetoelastic biosensor for the direct detection of organophosphorus pesticides
    Zourob, Mohammed
    Ong, Keat G.
    Zeng, Kefeng
    Mouffouk, Fouzi
    Grimes, Craig A.
    [J]. ANALYST, 2007, 132 (04) : 338 - 343