Peptidylarginine deiminase type I, type II, type III and type IV are expressed in rat epidermis

被引:26
作者
Ishigami, A
Asaga, H
Ohsawa, T
Akiyama, K
Maruyama, N
机构
[1] Tokyo Metropolitan Inst Gerontol, Dept Bioact Regulat, Itabashi Ku, Tokyo 1730015, Japan
[2] Tokyo Metropolitan Inst Gerontol, Dept Mol Pathol, Itabashi Ku, Tokyo 1730015, Japan
来源
BIOMEDICAL RESEARCH-TOKYO | 2001年 / 22卷 / 01期
关键词
D O I
10.2220/biomedres.22.63
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Peptidylarginine deiminases (PADs) are post-translational modification enzymes to convert protein arginine to citrulline residues in a calcium ion-dependent manner. Here, we investigated the tissue specific expression of PAD type T, II, III and IV by using a reverse transcription-polymerase chain reaction (RT-PCR) method. The type I mRNA was detected only in the epidermis and stomach, and type III mRNA was detected only in the epidermis and ovary. On the other hand, the type II and type IV mRNAs were found in a variety of tissues such as the epidermis, lung, spleen, stomach, kidney, ovary and uterus. The epidermis is the only tissue in which all four PAD mRNAs were detected. The present study suggests strongly that PADs play functionally important roles during the terminal differentiation of epidermal keratinocytes.
引用
收藏
页码:63 / 65
页数:3
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