Operon structure and gene expression of the espJ-tccP locus of enterohaernorrhagic Escherichia coli O157:H7

被引：17

作者：

Garmendia, J ^{[1
]}

Frankel, G ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, Ctr Mol Microbiol & Infect, London SW7 2AZ, England

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 247卷 / 02期

基金：

英国惠康基金;

关键词：

EHEC; effectors; type III secretion system; environmental signals; gene expression;

D O I：

10.1016/j.femsle.2005.04.035

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study, we investigated the genetic organisation and expression of the espJ-tccP locus of enterohaemorrhagic Escherichia coli (EHEC O157:H7), which encodes the type III secretion system effector proteins EspJ and TccP. Using flow cytometry and fluorescence microscopy, we found that espJ and tccP constitute an operon, whose expression in vitro is affected by the composition of the medium and environmental signals such as temperature, pH, osmolarity and 02 pressure. Moreover, expression of the espJ-tccP operon is not regulated by Ler, a LEE-encoded transcriptional regulator, but by an as yet unidentified regulatory system(s). (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

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页码：137 / 145

页数：9

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