A family of genes located on four separate 32-kilobase circular plasmids in Borrelia burgdorferi B31

被引：121

作者：

Stevenson, B

Tilly, K

Rosa, PA

机构：

[1] Lab. of Microbial Struct. and Funct., Rocky Mountain Laboratories, Natl. Inst. Allerg. and Infect. Dis., Hamilton, MT

[2] Lab. of Microbial Struct. and Funct., Rocky Mountain Laboratories, NIH, Hamilton, MT 59840

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 12期

关键词：

D O I：

10.1128/jb.178.12.3508-3516.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have identified four loci Borrelia burgdorferi B31 that contain open reading frames capable of encoding six proteins that are related to the antigenic proteins OspE and OspF. We have designated these proteins Erp, for OspEF-related protein, and named their respective genes erp. The erpA erpB genes are linked, as ar erpC and erpD, and the pairs probably constitute tow operons. The erpG and erpH genes appear to be monocistronic. The ErpA and ErpC proteins are expressed by B. burgdorferi B31 in culture and are recognized by polyclonal antiserum raised against the OspE protein of B. burgdorferi N40. The four erp loci are ach located on different 32-kb circular plasmids that contain additional DNA sequences that are homologous to each other and to an 8.3-kb circular plasmid of B. burgdorferi sensu lato Ip21. All four 32-kb plasmids can be maintained within a single bacterium, which may provide a model for the study of plasmid replication and segregation in B. burgdorferi.

引用

页码：3508 / 3516

页数：9

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