Highly branched stimuli responsive poly [(N-isopropyl acrylamide)-co-(1,2-propandiol-3-methacrylate)]s with protein binding functionality

被引：64

作者：

Carter, S

Rimmer, S

Sturdy, A

Webb, M

机构：

[1] Univ Sheffield, Dept Chem, Polymer & Biomat Chem Labs, Ctr Polymer, Sheffield S3 7HF, S Yorkshire, England

[2] Univ Manchester, Dept Med Genet, Ctr Mol Med, Manchester M13 9PT, Lancs, England

来源：

MACROMOLECULAR BIOSCIENCE | 2005年 / 5卷 / 05期

关键词：

affinity precipitation; copolymerization; His-tagged protein; reversible addition-fragmentation chain transfer (RAFT); water-soluble polymers;

D O I：

10.1002/mabi.200400218

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Highly branched poly(NIPAM) have been prepared using the technique of reversible addition-fragmentation chain transfer (RAFT) polymerisation using a chain transfer agent that allows the incorporation of imidazole functionality in the polymer chain-ends. The lower critical solution temperature (LCST) of the polymers can be controlled by the amount of hydrophobe and GMA incorporated during copolymerisation procedures. These thermally responsive "smart" polymers were used to purify a His-tagged BRCA-1 protein fragment by affinity precipitation.

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收藏

页码：373 / 378

页数：6

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