Single-cell genomics

被引：204

作者：

Kalisky, Tomer ^{[1
]}

Quake, Stephen R. ^{[1
,2
,3
]}

机构：

[1] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Appl Phys, Stanford, CA 94305 USA

[3] Howard Hughes Med Inst, Chevy Chase, MD USA

来源：

NATURE METHODS | 2011年 / 8卷 / 04期

关键词：

GENE-EXPRESSION; AMPLIFICATION; PCR; TRANSCRIPTOME; POLYMERASE;

D O I：

10.1038/nmeth0411-311

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods for genomic analysis at single-cell resolution enable new understanding of complex biological phenomena. Single-cell techniques, ranging from flow cytometry and microfluidics to PCR and sequencing, are used to understand the cellular composition of complex tissues, find new microbial species and perform genome-wide haplotyping. © 2011 Nature America, Inc. All rights reserved.

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页码：311 / 314

页数：4

相关论文

共 35 条

[1] Mass Cytometry: Technique for Real Time Single Cell Multitarget Immunoassay Based on Inductively Coupled Plasma Time-of-Flight Mass Spectrometry [J].

Bandura, Dmitry R. ;

Baranov, Vladimir I. ;

Ornatsky, Olga I. ;

Antonov, Alexei ;

Kinach, Robert ;

Lou, Xudong ;

Pavlov, Serguei ;

Vorobiev, Sergey ;

Dick, John E. ;

Tanner, Scott D. .

ANALYTICAL CHEMISTRY, 2009, 81 (16) :6813-6822

[2] Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels [J].

Bengtsson, M ;

Ståhlberg, A ;

Rorsman, P ;

Kubista, M .

GENOME RESEARCH, 2005, 15 (10) :1388-1392

[3] Genome of a Low-Salinity Ammonia-Oxidizing Archaeon Determined by Single-Cell and Metagenomic Analysis [J].

Blainey, Paul C. ;

Mosier, Annika C. ;

Potanina, Anastasia ;

Francis, Christopher A. ;

Quake, Stephen R. .

PLOS ONE, 2011, 6 (02)

[4] Digital MDA for enumeration of total nucleic acid contamination [J].

Blainey, Paul C. ;

Quake, Stephen R. .

NUCLEIC ACIDS RESEARCH, 2011, 39 (04) :e19

[5] Single-cell transcript analysis of pancreas development [J].

Chiang, MK ;

Melton, DA .

DEVELOPMENTAL CELL, 2003, 4 (03) :383-393

[6] Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed rolling circle amplification [J].

Dean, FB ;

Nelson, JR ;

Giesler, TL ;

Lasken, RS .

GENOME RESEARCH, 2001, 11 (06) :1095-1099

[7] Association of reactive oxygen species levels and radioresistance in cancer stem cells [J].

Diehn, Maximilian ;

Cho, Robert W. ;

Lobo, Neethan A. ;

Kalisky, Tomer ;

Dorie, Mary Jo ;

Kulp, Angela N. ;

Qian, Dalong ;

Lam, Jessica S. ;

Ailles, Laurie E. ;

Wong, Manzhi ;

Joshua, Benzion ;

Kaplan, Michael J. ;

Wapnir, Irene ;

Dirbas, Frederick M. ;

Somlo, George ;

Garberoglio, Carlos ;

Paz, Benjamin ;

Shen, Jeannie ;

Lau, Sean K. ;

Quake, Stephen R. ;

Brown, J. Martin ;

Weissman, Irving L. ;

Clarke, Michael F. .

NATURE, 2009, 458 (7239) :780-U123

[8] ANALYSIS OF GENE-EXPRESSION IN SINGLE LIVE NEURONS [J].

EBERWINE, J ;

YEH, H ;

MIYASHIRO, K ;

CAO, YX ;

NAIR, S ;

FINNELL, R ;

ZETTEL, M ;

COLEMAN, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) :3010-3014

[9] Stochastic gene expression in a single cell [J].

Elowitz, MB ;

Levine, AJ ;

Siggia, ED ;

Swain, PS .

SCIENCE, 2002, 297 (5584) :1183-1186

[10] Whole-genome molecular haplotyping of single cells [J].

Fan, H. Christina ;

Wang, Jianbin ;

Potanina, Anastasia ;

Quake, Stephen R. .

NATURE BIOTECHNOLOGY, 2011, 29 (01) :51-+

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