Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays

被引：1200

作者：

Fierer, N

Jackson, JA

Vilgalys, R

Jackson, RB

机构：

[1] Duke Univ, Dept Biol, Durham, NC 27708 USA

[2] Duke Univ, Nicholas Sch Environm & Earth Sci, Durham, NC 27708 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 07期

关键词：

D O I：

10.1128/AEM.71.7.4117-4120.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure.

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页码：4117 / 4120

页数：4

相关论文

共 32 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2]

Barns SM, 1999, APPL ENVIRON MICROB, V65, P1731

[3] Molecular characterization of bacterial diversity in Lodgepole pine (Pinus contorta) rhizosphere soils from British Columbia forest soils differing in disturbance and geographic source [J].

Chow, ML ;

Radomski, CC ;

McDermott, JM ;

Davies, J ;

Axelrood, PE .

FEMS MICROBIOLOGY ECOLOGY, 2002, 42 (03) :347-357

[4]

Christensen M., 1981, FUNGAL COMMUNITY ITS, P201

[5] The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy [J].

Cole, JR ;

Chai, B ;

Marsh, TL ;

Farris, RJ ;

Wang, Q ;

Kulam, SA ;

Chandra, S ;

McGarrell, DM ;

Schmidt, TM ;

Garrity, GM ;

Tiedje, JM .

NUCLEIC ACIDS RESEARCH, 2003, 31 (01) :442-443

[6]

Dunbar J, 1999, APPL ENVIRON MICROB, V65, P1662

[7] ITS PRIMERS WITH ENHANCED SPECIFICITY FOR BASIDIOMYCETES - APPLICATION TO THE IDENTIFICATION OF MYCORRHIZAE AND RUSTS [J].

GARDES, M ;

BRUNS, TD .

MOLECULAR ECOLOGY, 1993, 2 (02) :113-118

[8] The magnitude of fungal diversity: the 1.5 million species estimate revisited [J].

Hawksworth, DL .

MYCOLOGICAL RESEARCH, 2001, 105 :1422-1432

[9] A simple, efficient method for the separation of humic substances and DNA from environmental samples [J].

Jackson, CR ;

Harper, JP ;

Willoughby, D ;

Roden, EE ;

Churchill, PF .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (12) :4993-4995

[10] Quantitative measurement of fungal DNA extracted by three different methods using real-time polymerase chain reaction [J].

Kabir, S ;

Rajendran, N ;

Amemiya, T ;

Itoh, K .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2003, 96 (04) :337-343

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