Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays

被引：1200

作者：

Fierer, N

Jackson, JA

Vilgalys, R

Jackson, RB

机构：

[1] Duke Univ, Dept Biol, Durham, NC 27708 USA

[2] Duke Univ, Nicholas Sch Environm & Earth Sci, Durham, NC 27708 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 07期

关键词：

D O I：

10.1128/AEM.71.7.4117-4120.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure.

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收藏

页码：4117 / 4120

页数：4

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