Multitip scanning bio-Kelvin probe

被引：68

作者：

Baikie, ID ^{[1
]}

Smith, PJS ^{[1
]}

Porterfield, DM ^{[1
]}

Estrup, PJ ^{[1
]}

机构：

[1] Marine Biol Lab, Biocurrents Res Ctr, Woods Hole, MA 02543 USA

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 1999年 / 70卷 / 03期

关键词：

D O I：

10.1063/1.1149678

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We have developed a novel multitip scanning Kelvin probe which can measure changes in biological surface potential Delta V-s to within 2 mV and, quasisimultaneously monitor displacement to 1 mm. The control and measurement subcomponents are PC based and incorporate a flexible user interface permitting software control of each individual tip, measurement, and scan parameters. We review the mode of operation and design features of the scanning bio-Kelvin probe including tip steering, signal processing, tip calibration, and novel tip tracking/dithering routines. This system uniquely offers both tip-to-sample spacing control (which is essential to avoid spurious changes in Delta V-s due to variations in mean spacing) and a dithering routine to maintain tip orientation to the biological specimen, irrespective of the latter's movement. These features permit long term (>.48 h) "active'' tracking of the displacement and biopotentials developed along and around a plant shoot in response to an environmental stimulus, e.g., differential illumination (phototropism) or changes in orientation (gravitropism). (C) 1999 American Institute of Physics. [S0034-6748(99)04703-6].

引用

页码：1842 / 1850

页数：9

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