LC-MS for protein characterization: current capabilities and future trends

被引：35

作者：

Chen, Guodong ^{[1
]}

Pramanik, Birendra N. ^{[1
]}

机构：

[1] Schering Plough Res Inst, Kenilworth, NJ 07033 USA

来源：

EXPERT REVIEW OF PROTEOMICS | 2008年 / 5卷 / 03期

关键词：

bottom-up; CID; collision-induced dissociation; ECD; electron-capture dissociation; electron-transfer dissociation; ETD; LC-MS; liquid chromatography-mass spectrometry; peptide mass fingerprinting; top-down; ultra-high-performance liquid chromatography; ultra-HPLC;

D O I：

10.1586/14789450.5.3.435

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

With advances in ionization methods and instrumentation, liquid chromatography (LC)/mass spectrometry (MS) has become a powerful technology for protein characterization. This review article will describe the general approaches on LC-MS analysis in protein characterization, including bottom-up and top-down strategies. Discussions will be given on characterization of recombinant proteins, and post-translational and protein modifications such as disulfide bonds, glycosylation and phosphorylation using LC-MS. New research directions in this area will also be presented to illustrate future prospects of LC-MS in protein characterization, including application to proteomics.

引用

收藏

页码：435 / 444

页数：10

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