Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles

被引：104

作者：

Chen, Qiang ^{[1
]}

Tang, Wei ^{[1
]}

Wang, Dingzhong ^{[1
]}

Wu, Xiaojie ^{[1
]}

Li, Na ^{[1
]}

Liu, Feng ^{[1
]}

机构：

[1] Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2010年 / 26卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Aptamer; Gold nanoparticles; Amplification; QCM-D biosensor; Human alpha-thrombin; DNA; BIND; RNA; MOLECULES; SELECTION;

D O I：

10.1016/j.bios.2010.07.034

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A highly sensitive quartz crystal microbalance with dissipation monitoring (QCM-D) biosensor for protein was developed using aptamer-functionalized gold nanoparticles (Apt-GNPs) for amplification. Human alpha-thrombin, an important physiological protease found in blood, was chosen as the target protein. Captured by immobilized aptamers, thrombin was determined on-line using Apt-GNPs to enhance both frequency and dissipation signals. The fabricated sandwich of aptamer/thrombin/Apt-GNPs on chip surface was confirmed by atomic force microscopy (AFM). Compared to direct assay, the detection limit for thrombin was down to 0.1 nM, yielding about 2 orders of magnitude improvement in sensitivity. This aptamer-based QCM-D biosensor also showed good selectivity and repeatability in complex matrix. For the first time, the dual-signal enhancement of Apt-GNPs on QCM-D sensing was demonstrated, and such design could provide a promising detection strategy for proteins with two binding sites. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：575 / 579

页数：5

共 29 条

[1] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].