In Vivo Bioluminescent Imaging (BLI): Noninvasive Visualization and Interrogation of Biological Processes in Living Animals

被引:142
作者
Close, Dan M. [1 ]
Xu, Tingting [1 ]
Sayler, Gary S. [1 ]
Ripp, Steven [1 ]
机构
[1] Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
bacterial luciferase; bioluminescent imaging; BLI; firefly luciferase; Lux; Luc; MESENCHYMAL STEM-CELLS; PROTEIN-PROTEIN INTERACTIONS; INDUCIBLE FACTOR 1-ALPHA; FIREFLY LUCIFERASE; GENE-EXPRESSION; STREPTOCOCCUS-PNEUMONIAE; TOXOPLASMA-GONDII; WOUND INFECTIONS; ENERGY-TRANSFER; REPORTER MOUSE;
D O I
10.3390/s110100180
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In vivo bioluminescent imaging (BLI) is increasingly being utilized as a method for modern biological research. This process, which involves the noninvasive interrogation of living animals using light emitted from luciferase-expressing bioreporter cells, has been applied to study a wide range of biomolecular functions such as gene function, drug discovery and development, cellular trafficking, protein-protein interactions, and especially tumorigenesis, cancer treatment, and disease progression. This article will review the various bioreporter/biosensor integrations of BLI and discuss how BLI is being applied towards a new visual understanding of biological processes within the living organism.
引用
收藏
页码:180 / 206
页数:27
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