Rapid identification of Lactobacillus brevis using the polymerase chain reaction

被引:48
作者
Guarneri, T [1 ]
Rossetti, L [1 ]
Giraffa, G [1 ]
机构
[1] Ist Sperimentale Lattiero Caseario, Dept Microbiol & Enzymol, I-26900 Lodi, Italy
关键词
D O I
10.1046/j.1472-765X.2001.01014.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: Species-specific PCR was applied to identify Lactobacillus brevis and the sensitivity and the specificity of the protocol were determined. Methods and Results: Strains of Lact. brevis obtained from foods, particularly dairy products, and various strain collections, were identified by PCR using primers which amplified a 1340 by fragment within the 16S rRNA gene. The PCR product was obtained after amplification of all the Lact. brevis strains tested; the size of the amplicon was as expected. No PCR products were observed after amplification from DNA of several lactic acid bacteria (LAB) species. Conclusions: A PCR method was optimized to identify Lact. brevis. The protocol was highly efficient and sensitive. Significance and Impact of the Study: Conventional phenotypic methods often lead to ambiguous identification of LAB species belonging to Lact. brevis. The proposed protocol is sensitive, specific, and can be applied to total DNA extracted by use of chelating matrix with loss of neither sensitivity nor specificity.
引用
收藏
页码:377 / 381
页数:5
相关论文
共 14 条
[1]   Molecular diversity of lactic acid bacteria from cassava sour starch (Colombia) [J].
Ben Omar, N ;
Ampe, F ;
Raimbault, M ;
Guyot, JP ;
Tailliez, P .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2000, 23 (02) :285-291
[2]  
Bizzaro R, 2000, ITAL J FOOD SCI, V12, P303
[3]   Phenotypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat (species Triticum durum and Triticum aestivum) sourdoughs of Southern Italy [J].
Corsetti, A ;
Lavermicocca, P ;
Morea, M ;
Baruzzi, F ;
Tosti, N ;
Gobbetti, M .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 64 (1-2) :95-104
[4]   Biochemical and microbiological characterization of artisanal 'Penamellera' cheese:: analysis of its indigenous lactic acid bacteria [J].
Estepar, J ;
Sánchez, MD ;
Alonso, L ;
Mayo, B .
INTERNATIONAL DAIRY JOURNAL, 1999, 9 (10) :737-746
[5]   Note:: Identification of Lactobacillus delbrueckii subspecies bulgaricus and subspecies lactis dairy isolates by amplified rDNA restriction analysis [J].
Giraffa, G ;
De Vecchi, P ;
Rossetti, L .
JOURNAL OF APPLIED MICROBIOLOGY, 1998, 85 (05) :918-924
[6]  
Kandler O., 1986, BERGEYS MANUAL SYSTE, VII, P1209
[7]   IDENTIFICATION OF MESOPHILIC LACTIC-ACID BACTERIA BY USING POLYMERASE CHAIN REACTION-AMPLIFIED VARIABLE REGIONS OF 16S RIBOSOMAL-RNA AND SPECIFIC DNA PROBES [J].
KLIJN, N ;
WEERKAMP, AH ;
DEVOS, WM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (11) :3390-3393
[8]   SPECIATION OF LACTIC-ACID BACTERIA FROM WINES BY HYBRIDIZATION WITH DNA PROBES [J].
LONVAUDFUNEL, A ;
FREMAUX, C ;
BITEAU, N ;
JOYEUX, A .
FOOD MICROBIOLOGY, 1991, 8 (03) :215-222
[9]  
Marchesi JR, 1998, APPL ENVIRON MICROB, V64, P795
[10]  
POT B, 1994, BACTERIOCINS LACTIC, P55