Sec6 mutations and the Drosophila exocyst complex

被引：52

作者：

Murthy, M

Ranjan, R

Denef, N

Higashi, MEL

Schupbach, T

Schwarz, TL ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Childrens Hosp, Div Neurosci, Boston, MA 02115 USA

[2] Princeton Univ, Dept Mol Biol, Howard Hughes Med Inst, Princeton, NJ 08544 USA

来源：

JOURNAL OF CELL SCIENCE | 2005年 / 118卷 / 06期

关键词：

Sec6; Sec5; Sec8; oogenesis; membrane trafficking;

D O I：

10.1242/jcs.01644

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function.

引用

页码：1139 / 1150

页数：12

共 32 条

[1] The Rho GTPase Rho3 has a direct role in exocytosis that is distinct from its role in actin polarity [J].