Fluorimetric determination of hemoglobin using spiro form rhodamine B hydrazide in a micellar medium

被引：43

作者：

Yang, XF

Guo, XQ

Li, A ^{[1
]}

机构：

[1] NW Univ Xian, Inst Analyt Sci, Xian 710069, Peoples R China

[2] Xiamen Univ, Dept Chem, Xiamen 361005, Peoples R China

[3] Xiamen Univ, Key Lab Analyt Sci MOE, Xiamen 361005, Peoples R China

来源：

TALANTA | 2003年 / 61卷 / 04期

基金：

中国国家自然科学基金;

关键词：

rhodamine B hydrazide; SDBS; hemoglobin; fluorimetry;

D O I：

10.1016/S0039-9140(03)00306-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel fluorimetric method for the determination of hemoglobin (Hb) using spiro form rhodamine B hydrazide (RBH) as fluorogenic reagent in the presence of sodium dodecylbenzene sulfonate (SDBS) surfactant micelles is described. The method employs the reaction of Hb with RBH, a colorless, non-fluorescent spirolactam hydrazide in SDBS micelles to generate highly fluorescent product rhodamine B and hence leads to a large increase in fluorescence intensity. The fluorescence increase is linearly related to the concentration of Hb in the range 0.2-12.0 nmol l(-1) with a detection limit of 0.086 nmol l(-1) (3sigma). The optimal conditions for the detection of Hb are evaluated and the possible detection mechanism is also discussed. The proposed method has been applied to the determination of Hb in human blood. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：439 / 445

页数：7

共 18 条

[1] Electrochemical studies of rutin interacting with hemoglobin and determination of hemoglobin [J].