Poly(methylene blue) modified electrode sensor for haemoglobin

被引:93
作者
Brett, CMA [1 ]
Inzelt, G
Kertesz, V
机构
[1] Univ Coimbra, Dept Quim, P-3049 Coimbra, Portugal
[2] Eotvos Lorand Univ, Dept Phys Chem, Budapest 112, Hungary
关键词
poly(methylene blue); modified electrodes; haemoglobin; electrochemical sensor; batch injection analysis;
D O I
10.1016/S0003-2670(98)00808-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Poly(methylene blue) modified electrodes have been prepared by potential cycling from aqueous electrolyte solution containing methylene blue on glassy carbon substrates. They have been used successfully for the determination of haemoglobin via its electrooxidation at fixed potential using the batch injection analysis technique in which whole blood samples diluted with phosphate or acetate buffer are injected directly over the modified electrode immersed in inert electrolyte, Electrode response is fast, stable, and reproducible. For blood from healthy donors, a good linear correlation was found between the results from the electrochemical injection method and the: conventional clinical spectrophotometric measurement after cyanidation. The blood from potentially ill donors showed deviations from the Linear correlation suggesting that this may be a good method for detecting haematological problems. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:119 / 123
页数:5
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