Evidence of different metabolic phenotypes in humans

被引:202
作者
Assfalg, Michael [2 ]
Bertini, Ivano [1 ,4 ]
Colangiuli, Donato [3 ,5 ]
Luchinat, Claudio [1 ,6 ]
Schaefer, Hartmut [7 ]
Schuetz, Birk [7 ]
Spraul, Manfred [7 ]
机构
[1] Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy
[2] ProtEra, I-50019 Sesto Fiorentino, Italy
[3] Univ Florence, Ctr Metab, I-50019 Sesto Fiorentino, Italy
[4] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy
[5] FiorGen Fdn, I-50019 Sesto Fiorentino, Italy
[6] Univ Florence, Dept Agr Biotechnol, I-50144 Florence, Italy
[7] Bruker BioSpin, D-76287 Rheinstetten, Germany
关键词
biofluids; metabolomics; metabonomics; NMR; urine;
D O I
10.1073/pnas.0705685105
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The study of metabolic responses to drugs, environmental changes, and diseases is a new promising area of metabonomic research. Metabolic fingerprints can be obtained by analytical techniques such as nuclear magnetic resonance (NMR). In principle, alterations of these fingerprints due to appearance/disappearance or concentration changes of metabolites can provide early evidences of, for example, onset of diseases. A major drawback in this approach is the strong day-to-day variability of the individual metabolic fingerprint, which should be rather called a metabolic "snapshot." We show. here that a thorough statistical analysis performed on NMR spectra of human urine samples reveals an invariant part characteristic of each person, which can be extracted from the analysis of multiple samples of each single subject. This finding (i) provides evidence that individual metabolic phenotypes may exist and (ii) opens new perspectives to metabonomic studies, based on the possibility of eliminating the daily "noise" by multiple sample collection.
引用
收藏
页码:1420 / 1424
页数:5
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