The importance of "like to like" ISI calibrations with freeze dried plasmas

被引:13
作者
Poller, L
van den Besselaar, AMHP
Jespersen, J
Tripodi, A
Houghton, D
机构
[1] Univ Manchester, Dept Pathol Sci, Cent Facil, ECAA, Manchester M13 9PT, Lancs, England
[2] Leiden Univ, Med Ctr, Haemostasis & Thrombosis Res Ctr, NL-2300 RA Leiden, Netherlands
[3] Cent Sygehuset, Dept Clin Biochem, Esbjerg, Denmark
[4] Univ Milan, Haemophilia & Thrombosis Ctr, Milan, Italy
[5] Maggiore Hosp, IRCCS, Milan, Italy
关键词
thromboplastin; international sensitivity index; freeze dried plasma; species specificity;
D O I
10.1136/jcp.51.4.275
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Aim-To assess reliability of like to like and cross species calibrations using two types of certified freeze dried plasma calibrants-artificially depleted of vitamin K clotting factors, and from coumarin treated patients. Methods-Six ECAA national control laboratories provided certified values for the freeze dried plasmas in terms of the human plain international reference preparation (IRP) (BCT/441) with the manual prothrombin time technique. Eight other ECAA national laboratories determined international sensitivity index (ISI) values in full fresh plasma same species and cross species WHO calibrations against a low ISI human IRP (BCT/441) of the ECAA low ISI human thromboplastin and high ISI ECAA rabbit thromboplastin. Parallel calibrations were performed using the certified values. Results-Calibrations on fresh plasmas of the human ECAA reference thromboplastin (stated ISI = 0.95) gave ISI of 0.957 against the human IRP and 1.66 against the rabbit IRP. The ECAA rabbit (stated ISI = 1.67) gave an identical value on the fresh plasma calibration v the human IRP. With freeze dried depleted plasmas certified in terms of the human IRP, the ISI of the ECAA human was 1.01, but the ECAA rabbit (stated ISI = 1.67) gave a low ISI of 1.47. The freeze dried coumarin plasmas gave an ISI of 0.943 for the ECAA human but only 1.493 for the ECAA rabbit. Conclusions-Fresh plasmas give reliable ISI when calibrating thromboplastins in same species and cross species calibrations. Freeze dried plasmas certified in terms of a single IRP, whether artificially depleted or of coumarin plasma origin, cannot be used for calibration of dissimilar thromboplastins.
引用
收藏
页码:275 / 279
页数:5
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