Design and efficient synthesis of novel haptens and complete antigens for the AOZ, a toxic metabolite of furazolidone

被引：15

作者：

Shen, Yu Dong ^{[1
]}

Wang, Yu ^{[1
]}

Zhang, Shi Wei ^{[1
]}

Xiao, Zhi Li ^{[1
]}

Sun, Yuan Ming ^{[1
]}

Bu, Xian Zhang ^{[2
]}

Gu, Lian Quan ^{[2
]}

机构：

[1] S China Agr Univ, Food Qual & Safety Key Lab, Dept Educ Guangdong Prov, Inst Food Qual & Safety, Guangzhou 510642, Peoples R China

[2] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510080, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2007年 / 18卷 / 12期

关键词：

furazolidone; AOZ; antigen; hapten; immuncoassay;

D O I：

10.1016/j.cclet.2007.10.030

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A good strategy was brought forward for designing efficient haptens and complete antigens for 3-amino-2-oxazolidinone (AOZ). Haptens designed newly were achieved facilely in good yield by using LiCl-N(Et)(3) as new catalysis system, the structure of which was elucidated by spectroscopy analysis, such as NMR and MS. Novel antigens for AOZ were prepared successfully by convenient active ester method. The ratios of haptens 3 and 4 to carrier proteins were proven respectively as 41:1 (5a), 39:1 (6a), 11: 1 (5b) and 9:1 (6b) by trinitrobenzene sulfonic acid (TNBS) method. The results of indirect competitive ELISA (ic-ELISA) of antiserums indicated that the haptens with a short unsaturated side chain can evoke specific immune response effectively. (c) 2007 Yu Dong Shen. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

引用

页码：1490 / 1492

页数：3

共 9 条

[1] Use of phosphonic acid as a generic hapten in the production of broad specificity anti-organophosphate pesticide antibody [J].