Formation and stability of a Janus-Wedge type of DNA triplex

被引：36

作者：

Chen, DL ^{[1
]}

Meena ^{[1
]}

Sharma, SK ^{[1
]}

McLaughlin, LW ^{[1
]}

机构：

[1] Boston Coll, Merkert Chem Ctr, Dept Chem, Chestnut Hill, MA 02467 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 01期

关键词：

D O I：

10.1021/ja038081x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new type of DNA targeting with the formation of a Janus-Wedge (J-W) triple helix is described. The "wedge" residue (W) attached to a PNA backbone is designed to insert itself into double-stranded DNA and base pair with both Watson-Crick faces. To study the stability of such an assembly, we have examined the formation of the J-W triplex with dC8 - T8 target sequence. The use of this target sequence permits the study of this new helix form without competing Watson-Crick interactions between the two target residues. Studies indicate that the W strand binds to both target strands, with defined polarity and a stability (-15.2 kcal/mol) that is roughly the sum of the two independent duplex interactions. Copyright © 2004 American Chemical Society.

引用

页码：70 / 71

页数：2

共 18 条

[1]

BERVERS S, 2000, J AM CHEM SOC, V122, P5905

[2] JANUS WEDGES: A new approach towards nucleobase-pair recognition [J].

Branda, N ;

Kurz, G ;

Lehn, JM .

CHEMICAL COMMUNICATIONS, 1996, (21) :2443-2444

[3] SEQUENCE-SPECIFIC SCISSION OF RNA BY 1,10-PHENANTHROLINE COPPER LINKED TO DEOXYOLIGONUCLEOTIDES [J].

CHEN, CHB ;

SIGMAN, DS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (19) :6570-6572

[4] SITE-SPECIFIC OLIGONUCLEOTIDE BINDING REPRESSES TRANSCRIPTION OF THE HUMAN C-MYC GENE INVITRO [J].

COONEY, M ;

CZERNUSZEWICZ, G ;

POSTEL, EH ;

FLINT, SJ ;

HOGAN, ME .

SCIENCE, 1988, 241 (4864) :456-459

[5] SEQUENCE-SPECIFIC RECOGNITION OF THE MAJOR GROOVE OF DNA BY OLIGODEOXYNUCLEOTIDES VIA TRIPLE HELIX FORMATION - FOOTPRINTING STUDIES [J].

FRANCOIS, JC ;

SAISONBEHMOARAS, T ;

HELENE, C .

NUCLEIC ACIDS RESEARCH, 1988, 16 (24) :11431-11440

[6] Synthesis and triplex forming properties of pyrimidine derivative containing extended functionality [J].

Gianolio, DA ;

McLaughlin, LW .

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 1999, 18 (08) :1751-1769

[7] Towards mixed sequence recognition by triple helix formation [J].

Gowers, DM ;

Fox, KR .

NUCLEIC ACIDS RESEARCH, 1999, 27 (07) :1569-1577

[8] RECOGNITION OF THYMINE.ADENINE BASE-PAIRS BY GUANINE IN A PYRIMIDINE TRIPLE HELIX MOTIF [J].

GRIFFIN, LC ;

DERVAN, PB .

SCIENCE, 1989, 245 (4921) :967-971

[9] RECOGNITION OF MIXED-SEQUENCE DUPLEX DNA BY ALTERNATE-STRAND TRIPLE-HELIX FORMATION [J].

HORNE, DA ;

DERVAN, PB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (06) :2435-2437

[10] OLIGONUCLEOTIDE-DIRECTED TRIPLE HELIX FORMATION AT ADJACENT OLIGOPURINE AND OLIGOPYRIMIDINE DNA TRACTS BY ALTERNATE STRAND RECOGNITION [J].

JAYASENA, SD ;

JOHNSTON, BH .

NUCLEIC ACIDS RESEARCH, 1992, 20 (20) :5279-5288

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