Gold nanoparticle-based competitive colorimetric assay for detection of protein-protein interactions

被引:129
作者
Tsai, CS [1 ]
Yu, TB [1 ]
Chen, CT [1 ]
机构
[1] Natl Taiwan Univ, Dept Chem, Taipei 10764, Taiwan
关键词
D O I
10.1039/b507237a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A gold nanoparticle-based competitive colorimetric assay uses the ensemble of Concanavalin ( ConA) and mannopyranoside-encapsulated gold nanoparticles (Man-GNPs) to identify the binding partners for ConA and the binding constants are determined based on the wavelength shifts.
引用
收藏
页码:4273 / 4275
页数:3
相关论文
共 21 条
[1]  
Birch J. R., 1995, MONOCLONAL ANTIBODIE
[2]   Raman dye-labeled nanoparticle probes for proteins [J].
Cao, YC ;
Jin, RC ;
Nam, JM ;
Thaxton, CS ;
Mirkin, CA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (48) :14676-14677
[3]  
Connors K. A., 1987, BINDING CONSTANTS ME
[4]  
de la Fuente JM, 2001, ANGEW CHEM INT EDIT, V40, P2258, DOI 10.1002/1521-3773(20010618)40:12<2257::AID-ANIE2257>3.0.CO
[5]  
2-S
[6]   FACTORS AFFECTING THE BINDING OF GLYCOPROTEINS TO CONCANAVALIN-A AND RICINUS-COMMUNIS AGGLUTININ AND THE DISSOCIATION OF THEIR COMPLEXES [J].
DULANEY, JT .
ANALYTICAL BIOCHEMISTRY, 1979, 99 (02) :254-267
[7]   Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].
Elghanian, R ;
Storhoff, JJ ;
Mucic, RC ;
Letsinger, RL ;
Mirkin, CA .
SCIENCE, 1997, 277 (5329) :1078-1081
[8]   Rapid, quantitative colorimetric detection of a lectin using mannose-stabilized gold nanoparticles [J].
Hone, DC ;
Haines, AH ;
Russell, DA .
LANGMUIR, 2003, 19 (17) :7141-7144
[9]   A comprehensive two-hybrid analysis to explore the yeast protein interactome [J].
Ito, T ;
Chiba, T ;
Ozawa, R ;
Yoshida, M ;
Hattori, M ;
Sakaki, Y .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (08) :4569-4574
[10]   What controls the melting properties of DNA-linked gold nanoparticle assemblies? [J].
Jin, RC ;
Wu, GS ;
Li, Z ;
Mirkin, CA ;
Schatz, GC .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (06) :1643-1654