Enantioseparation of some clinically used drugs by capillary electrophoresis using sulfated β-cyclodextrin as a chiral selector

The enantiomeric separation of 37 clinically used racemic basic drugs among 50 drugs was achieved using sulfated beta-cyclodextrin (5-beta-CD) as chiral selector at pH2.5 and in the reversed polarity mode. The results obtained in this study were different from the one obtained using neutral beta-CD and its derivatives as chiral selectors. Using S-beta-CD as chiral selector did not require the presence of the substructure 4H to achieve chiral separation as observed with beta-Cyclodextrin (beta-CD) and its derivatives since among the 37 separated drugs only 7 possess the 4H substructure. The chiral discrimination depends on the appropriate interaction between the analyte and the sulfated p-cyclodextrin.