Antioxidant enzyme activity and lipid peroxidation in liver and kidney of rats exposed to microcystin-LR administered intraperitoneally

被引:242
作者
Morena, I
Pichardo, S
Jos, A
Gómez-Amores, L
Mate, A
Vazquez, CM
Cameán, AM
机构
[1] Univ Sevilla, Fac Farm, Area Toxicol, E-41012 Seville, Spain
[2] Univ Sevilla, Fac Farm, Area Fisiol, E-41012 Seville, Spain
关键词
microcystin-LR; rat; oxidative stress; liver; kidney;
D O I
10.1016/j.toxicon.2004.11.001
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The effect of acute exposure of intraperitoneal injection of microcystin-LR (MCLR) on antioxidant enzymes and lipid peroxidation has been studied in liver and kidney of rats. Rats were treated with two doses, i.e. 100 and 150 mu g of pure MCLR/kg body weight or saline solution. The enzyme activities of glutathione peroxidase (GSH-Px), glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT) in the liver were significantly decreased in MCLR-treated rats. The decrease of GR activity in the liver was 60%, followed by GSH-Px, SOD and CAT. Similarly, a decrease in the antioxidant enzymes was found in the kidney of MCLR-treated rats, such as GSH-Px (27-31%), GR (22%), SOD (42%) and CAT (25-28%). Concomitantly, significant increases in lipid peroxidation levels were recorded in liver (121 and 196% for 100 and 150 mu g/kg, respectively) and kidney (48 and 58% for 100 and 150 mu g/kg, respectively) from MCLR-treated rats. In conclusion, acute exposure to MCLR results in a decrease in the antioxidant enzymes and an increase in lipid peroxidation in liver and kidney rats, suggesting the oxidative stress as an important role in the pathogenesis of MCLR-induced toxicity. Antioxidant enzymes were significantly consumed in the liver and a minor decrease was found in kidney, confirming the organ-specific effects of MCLR. (c) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:395 / 402
页数:8
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