Staphylococcus aureus virulence factors identified by using a high-throughput Caenorhabditis elegans-killing model

被引:63
作者
Begun, J
Sifri, CD
Goldman, S
Calderwood, SB
Ausubel, FM
机构
[1] Massachusetts Gen Hosp, Dept Biol Mol, Boston, MA 02114 USA
[2] Massachusetts Gen Hosp, Div Infect Dis, Boston, MA 02114 USA
[3] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
[4] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
关键词
D O I
10.1128/IAI.73.2.872-877.2005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Staphylococcus aureus is an important human pathogen that is also able to kill the model nematode Caenorhabditis elegans. We constructed a 2,950-member Tn917 transposon insertion library in S. aureus strain NCTC 8325. Twenty-one of these insertions exhibited attenuated C. elegans killing, and of these, 12 contained insertions in different genes or chromosomal locations. Ten of these 12 insertions showed attenuated killing phenotypes when transduced into two different S. aureus strains, and 5 of the 10 mutants correspond to genes that have not been previously identified in signature-tagged mutagenesis studies. These latter five mutants were tested in a murine renal abscess model, and one mutant harboring an insertion in nagD exhibited attenuated virulence. Interestingly, Tn917 was shown to have a very strong bias for insertions near the terminus of DNA replication.
引用
收藏
页码:872 / 877
页数:6
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共 44 条
[21]  
Iandolo JJ, 2000, GRAM-POSITIVE PATHOGENS, P317
[22]   Comparative analysis of the genomes of the temperate bacteriophages φ11, φ12 and φ13 of Staphylococcus aureus 8325 [J].
Iandolo, JJ ;
Worrell, V ;
Groicher, KH ;
Qian, YD ;
Tian, RY ;
Kenton, S ;
Dorman, A ;
Ji, HG ;
Lin, SP ;
Loh, P ;
Qi, SL ;
Zhu, H ;
Roe, BA .
GENE, 2002, 289 (1-2) :109-118
[23]   A Caenorhabditis elegans model of Yersinia infection:: biofilm formation on a biotic surface [J].
Joshua, GWP ;
Karlyshev, AV ;
Smith, MP ;
Isherwood, KE ;
Titball, RW ;
Wren, BW .
MICROBIOLOGY-SGM, 2003, 149 :3221-3229
[24]   NATURE OF DETERMINANT OF TETRACYCLINE RESISTANCE IN STAPHYLOCOCCUS AUREUS [J].
KASATIYA, SS ;
BALDWIN, JN .
CANADIAN JOURNAL OF MICROBIOLOGY, 1967, 13 (08) :1079-&
[25]   Whole genome sequencing of meticillin-resistant Staphylococcus aureus [J].
Kuroda, M ;
Ohta, T ;
Uchiyama, I ;
Baba, T ;
Yuzawa, H ;
Kobayashi, I ;
Cui, LZ ;
Oguchi, A ;
Aoki, K ;
Nagai, Y ;
Lian, JQ ;
Ito, T ;
Kanamori, M ;
Matsumaru, H ;
Maruyama, A ;
Murakami, H ;
Hosoyama, A ;
Mizutani-Ui, Y ;
Takahashi, NK ;
Sawano, T ;
Inoue, R ;
Kaito, C ;
Sekimizu, K ;
Hirakawa, H ;
Kuhara, S ;
Goto, S ;
Yabuzaki, J ;
Kanehisa, M ;
Yamashita, A ;
Oshima, K ;
Furuya, K ;
Yoshino, C ;
Shiba, T ;
Hattori, M ;
Ogasawara, N ;
Hayashi, H ;
Hiramatsu, K .
LANCET, 2001, 357 (9264) :1225-1240
[26]   Virulence factors of the human opportunistic pathogen Serratia marcescens identified by in vivo screening [J].
Kurz, CL ;
Chauvet, S ;
Andrès, E ;
Aurouze, M ;
Vallet, I ;
Michel, GPF ;
Uh, M ;
Celli, J ;
Filloux, A ;
de Bentzmann, S ;
Steinmetz, I ;
Hoffmann, JA ;
Finlay, BB ;
Gorvel, JP ;
Ferrandon, D ;
Ewbank, JJ .
EMBO JOURNAL, 2003, 22 (07) :1451-1460
[27]  
Lewis JA, 1995, METHOD CELL BIOL, V48, P3
[28]   Identification of novel staphylococcal virulence genes by in vivo expression technology [J].
Lowe, AM ;
Beattie, DT ;
Deresiewicz, RL .
MOLECULAR MICROBIOLOGY, 1998, 27 (05) :967-976
[29]   Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa Caenorhabditis elegans pathogenesis model [J].
Mahajan-Miklos, S ;
Tan, MW ;
Rahme, LG ;
Ausubel, FM .
CELL, 1999, 96 (01) :47-56
[30]   SELECTION OF BACTERIAL VIRULENCE GENES THAT ARE SPECIFICALLY INDUCED IN HOST TISSUES [J].
MAHAN, MJ ;
SLAUCH, JM ;
MEKALANOS, JJ .
SCIENCE, 1993, 259 (5095) :686-688