Column technology for capillary electrochromatography

被引:79
作者
Tang, QL [1 ]
Lee, ML [1 ]
机构
[1] Brigham Young Univ, Dept Biochem & Chem, Provo, UT 84602 USA
基金
美国国家科学基金会;
关键词
review; capillary electrochromatography; column technology; capillary columns; packed; open-tubular; continuous-bed; monolithic; polymeric; sol-gel; particle-fixed;
D O I
10.1016/S0165-9936(00)00048-0
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Column technologies for capillary electrochromatography( CEC) are reviewed. To achieve high efficiency, the inner diameters of open-tubular and packed columns should be less than 25 and 200 mum, respectively. To obtain acceptable separation speed under typical CEC conditions (e.g. 30 kV, 1 mm s(-1) electroosmotic flow velocity, and 2-4 x 10(-8) m(2) V-1 s(-1) electroosmotic mobility) the column lengths for open-tubular and packed columns should be less than 120 and 60 cm, respectively. Capillary CEC columns are generally classified into three types: packed, open-tubular, and continuous-bed or monolithic. The various column preparation procedures and the advantages and disadvantages of each column type are discussed in detail. (C) 2000 Published by Elsevier Science B.V.
引用
收藏
页码:648 / 663
页数:16
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