Improving the sensitivity of immunoassays by tuning gold nanoparticles to the tipping point

被引：11

作者：

Aveyard, Jenny ^{[1
]}

Nolan, Paul ^{[2
]}

Wilson, Robert ^{[1
]}

机构：

[1] Univ Liverpool, Dept Chem, Liverpool L69 7ZD, Merseyside, England

[2] Univ Liverpool, Dept Phys, Liverpool L69 7ZE, Merseyside, England

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 15期

关键词：

D O I：

10.1021/ac800699k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Conventional lateral flow immunoassays are based on labeled antibodies. In this paper we describe an alternative design based on gold nanoparticles labeled with haptens. The haptens are conjugated to gold nanoparticles by a method that allows the number per particle to be tuned to the point of maximum sensitivity. This leads to improvements compared with conventional lateral flow devices without relinquishing any of their advantages. In parallel assays for the environmental pollutant 2,4-dinitrophenol the alternative devices were 50% more sensitive.

引用

页码：6001 / 6005

页数：5

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