Live optical projection tomography

被引：38

作者：

Colas, Jean-Francois ^{[1
]}

Sharpe, James

机构：

[1] UPF, Ctr Genom Regulat, EMBL CRG Syst Biol Program, Barcelona, Spain

来源：

ORGANOGENESIS | 2009年 / 5卷 / 04期

关键词：

blood circulation; conceptus culture; ex vivo; ex utero; heartbeat; limb bud; live imaging; milieu interieur; optical tomography; organogenesis; GENE-EXPRESSION; MOUSE; EMBRYO; HEART; TOOL; QUANTIFICATION; MICROSCOPY; ANATOMY;

D O I：

10.4161/org.5.4.10426

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Optical projection tomography (OPT) is a technology ideally suited for imaging embryonic organs. We emphasize here recent successes in translating this potential into the field of live imaging. Live OPT (also known as 4D OPT, or time-lapse OPT) is already in position to accumulate good quantitative data on the developmental dynamics of organogenesis, a prerequisite for building realistic computer models and tackling new biological problems. Yet, live OPT is being further developed by merging state-of-the-art mouse embryo culture with the OPT system. We discuss the technological challenges that this entails and the prospects for expansion of this molecular imaging technique into a wider range of applications.

引用

页码：211 / 216

页数：6

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