In vitro whole-organ imaging:: 4D quantification of growing mouse limb buds

被引：62

作者：

Boot, Marit J. ^{[1
]}

Westerberg, C. Henrik ^{[2
]}

Sanz-Ezquerro, Juanjo ^{[3
]}

Cotterell, James ^{[1
,2
]}

Schweitzer, Ronen ^{[4
]}

Torres, Miguel ^{[3
]}

Sharpe, James ^{[1
,2
,5
]}

机构：

[1] Univ Edinburgh, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland

[2] Univ Pompeu Fabra, European Mol Biol Lab, Ctr Genom Regulat, Syst Biol Unit, Barcelona 08003, Spain

[3] Ctr Nacl Invest Cardiovasc, Madrid 28029, Spain

[4] Shriners Hosp Children, Portland, OR 97239 USA

[5] ICREA, Barcelona 08010, Spain

来源：

NATURE METHODS | 2008年 / 5卷 / 07期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1038/nmeth.1219

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).

引用

页码：609 / 612

页数：4

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