Molecular characterization of porcine picobirnaviruses and development of a specific reverse transcription-PCR assay

被引：32

作者：

Carruyo, Gabriela M. ^{[1
,2
]}

Mateu, Guaniri ^{[1
]}

Martinez, Laura C. ^{[3
]}

Pujol, Flor H. ^{[1
]}

Nates, Silvia V. ^{[3
]}

Liprandi, Ferdinando ^{[1
]}

Ludert, Juan E. ^{[1
]}

机构：

[1] Inst Venezolano Invest Cient, Ctr Microbiol & Biol Celular, Caracas, Venezuela

[2] La Univ Zulia, Fac Vet, Maracaibo, Venezuela

[3] Univ Nacl Cordoba, Fac Ciencias Med, RA-5000 Cordoba, Argentina

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 07期

关键词：

D O I：

10.1128/JCM.00655-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The molecular characterization of partial-length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (> 85% identity) to human PBV belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and Argentina showed that PBV circulate at high frequencies in piglets.

引用

页码：2402 / 2405

页数：4

共 30 条

[1] Diagnosis of noncultivatable gastroenteritis viruses, the human caliciviruses [J].

Atmar, RL ;

Estes, MK .

CLINICAL MICROBIOLOGY REVIEWS, 2001, 14 (01) :15-+

[2] Sequence heterogeneity among human picobirnaviruses detected in a gastroenteritis outbreak [J].

Baányai, K ;

Jakab, F ;

Reuter, G ;

Bene, J ;

Uj, M ;

Melegh, B ;

Szücs, G .

ARCHIVES OF VIROLOGY, 2003, 148 (12) :2281-2291

[3] Genogroup I picobirnaviruses in pigs:: evidence for genetic diversity and relatedness to human strains [J].

Banyai, K. ;

Martella, V. ;

Bogdan, A. ;

Forgach, P. ;

Jakab, F. ;

Meleg, E. ;

Biro, H. ;

Melegh, B. ;

Szucs, G. .

JOURNAL OF GENERAL VIROLOGY, 2008, 89 :534-539

[4] Detection of genogroup I and II human picobirnaviruses showing small genomic RNA profile causing acute watery diarrhoea among children in Kolkata, India [J].

Bhattacharya, Rittwika ;

Sahoo, Ganesh C. ;

Nayak, Mukti K. ;

Rajendran, K. ;

Dutta, P. ;

Mitra, Utpala ;

Bhattacharya, M. K. ;

Naik, T. N. ;

Bhattacharya, S. K. ;

Krishnan, Triveni .

INFECTION GENETICS AND EVOLUTION, 2007, 7 (02) :229-238

[5]

Bhattacharya R, 2006, INFECT GENET EVOL, V6, P453, DOI 10.1016/j.meegid.2006.02.005

[6] RELATIONSHIPS AMONG THE POSITIVE STRAND AND DOUBLE-STRAND RNA VIRUSES AS VIEWED THROUGH THEIR RNA-DEPENDENT RNA-POLYMERASES [J].

BRUENN, JA .

NUCLEIC ACIDS RESEARCH, 1991, 19 (02) :217-226

[7] Identification of a bisegmented double-stranded RNA virus (picobirnavirus) in calf faeces [J].

Buzinaro, MG ;

Freitas, PPS ;

Kisiellius, JJ ;

Ueda, M ;

Jerez, JA .

VETERINARY JOURNAL, 2003, 166 (02) :185-187

[8] Identification of picobirnavirus from faeces of Italian children suffering from acute diarrhea [J].

Cascio, A ;

Bosco, M ;

Vizzi, E ;

Giammanco, A ;

Ferraro, D ;

Arista, S .

EUROPEAN JOURNAL OF EPIDEMIOLOGY, 1996, 12 (05) :545-547

[9]

CHASEY D, 1990, VET REC, V126, P465

[10] DETECTION AND CHARACTERIZATION OF A NOVEL BISEGMENTED DOUBLE-STRANDED-RNA VIRUS (PICOBIRNAVIRUS) FROM RABBIT FECES [J].

GALLIMORE, C ;

LEWIS, D ;

BROWN, D .

ARCHIVES OF VIROLOGY, 1993, 133 (1-2) :63-73

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