The discrimination of IgM and IgG type antibodies and Fab' and F(ab)(2) antibody fragments on an industrial substrate using scanning force microscopy

被引：27

作者：

Roberts, CJ ^{[1
]}

Davies, MC ^{[1
]}

Tendler, SJB ^{[1
]}

Williams, PM ^{[1
]}

Davies, J ^{[1
]}

Dawkes, AC ^{[1
]}

Yearwood, GDL ^{[1
]}

Edwards, JC ^{[1
]}

机构：

[1] JOHNSON & JOHNSON CLIN DIAGNOST LTD,POLLARDS WOOD LABS,CHALFONT ST GILES HP8 4SP,BUCKS,ENGLAND

来源：

ULTRAMICROSCOPY | 1996年 / 62卷 / 03期

关键词：

D O I：

10.1016/0304-3991(95)00143-3

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We have previously employed scanning force microscopy (SFM) to study antibody-antigen molecular interactions on microtiter wells used for enzyme linked immunosorbant assays (ELISA). Here we demonstrate the ability of SFM to image and discriminate different types of antibody and antibody fragments bound to an ELISA well surface, The samples studied include a type IgG antibody with a proportion of bound IgM and two-dimensional films of whole IgG antibody, and Fab' and F(ab)(2) antibody fragments. Molecular resolution is achieved in each case despite the size of substrate features exceeding most of the molecular dimensions observed. Analysis of the data shows that the SFM overestimates molecular dimensions by an approximately constant amount, which is proposed to principally result from the effects of a finite probe size and not from deformation of the molecular species due to the imaging forces employed.

引用

页码：149 / 155

页数：7

共 30 条

[1] SCANNING TUNNELING MICROSCOPY OF RECA-DNA COMPLEXES COATED WITH A CONDUCTING FILM [J].

AMREIN, M ;

STASIAK, A ;

GROSS, H ;

STOLL, E ;

TRAVAGLINI, G .

SCIENCE, 1988, 240 (4851) :514-516

[2] 3-DIMENSIONAL STRUCTURE OF IMMUNOGLOBULINS [J].

AMZEL, LM ;

POLJAK, RJ .

ANNUAL REVIEW OF BIOCHEMISTRY, 1979, 48 :961-997

[3] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDY OF A CHIMERIC FAB' FRAGMENT OF ANTIBODY-BINDING TUMOR-CELLS [J].

BRADY, RL ;

HUBBARD, RE ;

KING, DJ ;

LOW, DC ;

ROBERTS, SM ;

TODD, RJ .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 219 (04) :603-604

[4] INTERPRETATION ISSUES IN FORCE MICROSCOPY [J].

BURNHAM, NA ;

COLTON, RJ ;

POLLOCK, HM .

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS, 1991, 9 (04) :2548-2556

[5]

CREIGHTON TE, 1989, PROTEIN STRUCTURE, P288

[6] USE OF SCANNING PROBE MICROSCOPY AND SURFACE-PLASMON RESONANCE AS ANALYTICAL TOOLS IN THE STUDY OF ANTIBODY-COATED MICROTITER WELLS [J].

DAVIES, J ;

ROBERTS, CJ ;

DAWKES, AC ;

SEFTON, J ;

EDWARDS, JC ;

GLASBEY, TO ;

HAYMES, AG ;

DAVIES, MC ;

JACKSON, DE ;

LOMAS, M ;

SHAKESHEFF, KM ;

TENDLER, SJB ;

WILKINS, MJ ;

WILLIAMS, PM .

LANGMUIR, 1994, 10 (08) :2654-2661

[7] A SCANNING-TUNNELING-MICROSCOPY COMPARISON OF PASSIVE ANTIBODY ADSORPTION AND BIOTINYLATED ANTIBODY LINKAGE TO STREPTAVIDIN ON MICROTITER WELLS [J].

DAVIES, J ;

DAWKES, AC ;

HAYMES, AG ;

ROBERTS, CJ ;

SUNDERLAND, RF ;

WILKINS, MJ ;

DAVIES, MC ;

TENDLER, SJB ;

JACKSON, DE ;

EDWARDS, JC .

JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 167 (1-2) :263-269

[8] DIRECT OBSERVATION OF DEFECT STRUCTURE IN PROTEIN CRYSTALS BY ATOMIC FORCE AND TRANSMISSION ELECTRON-MICROSCOPY [J].

DEVAUD, G ;

FURCINITTI, PS ;

FLEMING, JC ;

LYON, MK ;

DOUGLAS, K .

BIOPHYSICAL JOURNAL, 1992, 63 (03) :630-638

[9] INVESTIGATING POLY(1-BUTENE) FILMS BY SFM STM [J].

ENG, LM ;

FUCHS, H ;

JANDT, KD ;

PETERMANN, J .

ULTRAMICROSCOPY, 1992, 42 :989-997

[10] 3-DIMENSIONAL CONFORMATION OF GAMMAM AND GAMMA-A GLOBULIN MOLECULES [J].

FEINSTEIN, A ;

RICHARDSON, NE ;

MUNN, EA .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1971, 190 :104-+

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