Organic co-solvents restore the inherently high enantiomeric ratio of lipase B from Candida antarctica in hydrolytic resolution by relieving the enantiospecific inhibition of product alcohol

被引：23

作者：

Lundhaug, K

Overbeeke, PLA

Jongejan, JA

Anthonsen, T ^{[1
]}

机构：

[1] Norwegian Univ Sci & Technol, Dept Chem, N-7034 Trondheim, Norway

[2] Delft Univ Technol, Dept Biotechnol, NL-2628 BC Delft, Netherlands

来源：

TETRAHEDRON-ASYMMETRY | 1998年 / 9卷 / 16期

关键词：

D O I：

10.1016/S0957-4166(98)00273-0

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The enantiomeric ratio in the hydrolysis of racemic 3-chloro-1-phenylmethoxy-2-propyl butanoate with lipase B from Candida antarctica, CALB, is raised from E=50 to E=180 upon addition of acetone to the aqueous medium. This co-solvent effect is now explained as an enantiospecific inhibition of the lipase by the liberated alcohol. In the range from 0 to 30% acetone, the effect correlates with the increased solubility of the alcohol in the reaction medium. Free and immobilized CALB show similar behaviour. (C) 1998 Elsevier Science Ltd. All rights reserved.

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收藏

页码：2851 / 2856

页数：6

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